We narrowed to 39 results for: cas

1. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

   Plasmid Kit

   #1000000171

   Purpose

   Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

   Depositor

   Sylvestre Marillonnet, Johannes Stuttmann

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

2. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

   Plasmid Kit

   #1000000062

   Purpose

   Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit

   Depositor

   Takashi Yamamoto

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

3. EasyCloneYALI- CRISPR/Cas9 Set

   Plasmid Kit

   #1000000141

   Purpose

   Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9

   Depositor

   Irina Borodina

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

4. MoClo CRISPR/Cas Toolkit for Plants

   Plasmid Kit

   #1000000159

   Purpose

   The MoClo CRISPR/Cas Toolkit for Plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots.

   Depositor

   Vladimir Nekrasov

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

5. Multiplex CRISPR/Cas9 Assembly System Kit

   Plasmid Kit

   #1000000055

   Purpose

   Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack

   Depositor

   Takashi Yamamoto

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. CRISPR-Cas9 Gateway System for Physcomitrella patens

   Plasmid Kit

   #1000000151

   Purpose

   CRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.

   Depositor

   Magdalena Bezanilla

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

7. GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9

   Plasmid Kit

   #1000000154

   Purpose

   Vectors for integration using short homology and CRISPR/Cas9 to produce knockout alleles with and without a fluorescent reporter, fusion proteins with endogenous genes, and Cre drivers.

   Depositor

   Jeffrey Essner

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

8. The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

   Plasmid Kit

   #1000000210

   Purpose

   Toolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.

   Depositor

   Vincent Martin

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

9. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

   Plasmid Kit

   #1000000221

   Purpose

   A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

   Depositor

   Eric Cantor

   Available since

   June 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

10. GFP-itis Bacterial Base Editor Activity

    Plasmid Kit

    #1000000219

    Purpose

    This collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.

    Depositor

    Alexis Komor

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

11. CRISPi Kit

    Plasmid Kit

    #1000000136

    Purpose

    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.

    Depositor

    Brigitte Gasser

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

12. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit

    #1000000109

    Purpose

    Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.

    Depositor

    Bob Goldstein

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

13. SapTrap Kit

    Plasmid Kit

    #1000000077

    Purpose

    SapTrap: modular toolkit for building CRISPR/Cas9 targeting vectors to insert genetic tags in C. elegans genome. 21 vector assembly plasmids, Unc-32::GFP targeting vector, 5 Cre/Flp expression vectors

    Depositor

    Erik Jorgensen

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

14. CRISPRainbow Kit for Multiplex Labeling

    Plasmid Kit

    #1000000079

    Purpose

    CRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.

    Depositor

    Thoru Pederson

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCut plasmid toolkit

    Plasmid Kit

    #1000000118

    Purpose

    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.

    Depositor

    Aindrila Mukhopadhyay

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pDGE Dicot Genome Editing Kit

    Plasmid Kit

    #1000000084

    Purpose

    Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of “genome editing recipient” and “sgRNA shuttle” vectors.

    Depositor

    Johannes Stuttmann

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

17. CRISPaint Gene Tagging Kit

    Plasmid Kit

    #1000000086

    Purpose

    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

    Depositor

    Veit Hornung

    Available since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

18. CRISPRecise Kit

    Plasmid Kit

    #1000000235

    Purpose

    CRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.

    Depositor

    Ervin Welker

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

19. pMVP and pMAGIC Cloning Systems

    Plasmid Kit

    #1000000155

    Purpose

    pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.

    Depositor

    Christopher Newgard

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

20. OVC Extended Collection

    Plasmid Kit

    #1000000165

    Purpose

    Four plasmids containing the cloning cassette, eight plasmids containing LSDs engineered to heterodimerize and their binding partners, and five plasmids with different light-activated Opto-caspase 9.

    Depositor

    Harald Janovjak

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only