252,884 results

1. yTREX

   Plasmid

   #177306

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

   Depositor

   Karl-Erich Jaeger

   Article

   Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCL149

   Plasmid

   #134457

   Purpose

   CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.

   Depositor

   Thomas Bernhardt

   Article

   Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

   Insert

   Para-popZ-rbs-H3H4-msfGFPN-CTM.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Para

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHCL149

   Plasmid

   #134457

   Purpose

   CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.

   Depositor

   Thomas Bernhardt

   Article

   Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

   Insert

   Para-popZ-rbs-H3H4-msfGFPN-CTM.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Para

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUGW-H1-Scrambled control shRNA

   Plasmid

   #40625

   Depositor

   Sally Temple

   Article

   Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

   Insert

   Scrambled control shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUGW-H1-Scrambled control shRNA

   Plasmid

   #40625

   Depositor

   Sally Temple

   Article

   Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

   Insert

   Scrambled control shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TO-hNIL

   Plasmid

   #172113

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV.rTH.PI.Cre.SV40 (AAV Retrograde)

   Viral Prep

   #107788-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV.rTH.PI.Cre.SV40 (#107788). In addition to the viral particles, you will also receive purified AAV.rTH.PI.Cre.SV40 plasmid DNA. rTH promoter driving Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   rTH

   Tags

   None

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

8. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

   Plasmid

   #67974

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

   Plasmid

   #67974

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSuper-Retro-Puro-Drp1-shRNA

    Plasmid

    #99385

    Purpose

    Expresses shRNA against human Drp1 from puromycin resistance retroviral vector

    Depositor

    David Kashatus

    Article

    Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

    Insert

    Dynamin-1-like protein (DNM1L Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSuper-Retro-Puro-Drp1-shRNA

    Plasmid

    #99385

    Purpose

    Expresses shRNA against human Drp1 from puromycin resistance retroviral vector

    Depositor

    David Kashatus

    Article

    Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

    Insert

    Dynamin-1-like protein (DNM1L Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. OPEN HLA-A*02:01-BirA

    Plasmid

    #215569

    Purpose

    E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    BirA

    Expression

    Bacterial

    Mutation

    Glycine 120 to Cysteine

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HyPer7.2DAAO-NLS

    Plasmid

    #168302

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_SEPT2-msfGFP

    Plasmid

    #180318

    Purpose

    mammalian expression of human SEPT2 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR1a-tagRFP-KDEL

    Plasmid

    #114177

    Purpose

    Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Gateway entry clone

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR1a-tagRFP-KDEL

    Plasmid

    #114177

    Purpose

    Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Gateway entry clone

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT-MEK1-V5

    Plasmid

    #217297

    Purpose

    Lentivirus vector for expression of MEK1

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

    Plasmid

    #207505

    Purpose

    This plasmid can be used to generate retrovirus.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF, HA-GD2-28z_CAR (BATF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcVpx.myc

    Plasmid

    #213388

    Purpose

    Generate lentiviral vector which increases gene delivery into primary human myeloid cells

    Depositor

    Nathaniel Landau

    Article

    Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

    Insert

    SIVmac vpx (vpx Simian Immunodeficiency Virus)
    

    Use

    Lentiviral

    Tags

    myc-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SiC-V2-Cas9G7

    Plasmid

    #133043

    Purpose

    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    Tet repressor
    

    Use

    Lentiviral

    Tags

    Cerulean

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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