We narrowed to 18,457 results for: multi

1. pEU3-NII-GLICNot-C-His

   Plasmid

   #140187

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   6xHis and GST

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-51b(+) ChemoG 3.2

   Plasmid

   #193803

   Purpose

   Protein production of ChemoG3.2 in bacteria

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG 3.2
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   T7

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3'-3xFLAG minimal CMV pCDNA5

   Plasmid

   #212045

   Purpose

   LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck

   Plasmid

   #58524

   Purpose

   Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   sdChR(C138S, E154A)-TS-GCaMP6f-ER
   

   Use

   Lentiviral

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   changed Cysteine 138 to Serine; changed Glutamate…

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pActinin-miRFP670nano

   Plasmid

   #127432

   Purpose

   Alpha actinin labeling with near-infrared fluorescent protein

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   Alpha-actinin-miRFP670nano
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. RCAS-myc

   Plasmid

   #11548

   Depositor

   Sandra Orsulic

   Article

   Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

   Insert

   c-myc (MYC Human)
   

   Use

   Retroviral

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5/FRT/TO_Lifeact_STf

   Plasmid

   #182011

   Purpose

   CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   Lifeact_STf
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Salsa-BC15-P1B3

   Plasmid

   #202856

   Purpose

   Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15

   Depositor

   Noah Palm

   Article

   Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

   Insert

   EGFP/BC15
   

   Expression

   Mammalian

   Promoter

   Tet Response Element (TRE)

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Salsa-BC11-P1A11

   Plasmid

   #202852

   Purpose

   Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11

   Depositor

   Noah Palm

   Article

   Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

   Insert

   EGFP/BC11
   

   Expression

   Mammalian

   Promoter

   Tet Response Element (TRE)

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV/3' Box_(GLuc)_TOP2

    Plasmid

    #68435

    Purpose

    Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP2 construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-NLS-ZapCmR2

    Plasmid

    #59012

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR2 genetically encoded Zn(II) sensor
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pU6_(GLuc)_INT[Spinach2]

    Plasmid

    #68430

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Spinach2" aptamer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS4

    Plasmid

    #55751

    Purpose

    Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Expression

    Bacterial

    Mutation

    Residues 1-59 of KanR

    Promoter

    T7-lac

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMN-011

    Plasmid

    #195415

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF3

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF3 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMZ-314

    Plasmid

    #195459

    Purpose

    Lentiviral Integratable, ABA-inducible expression of synZiFTR: ZF1-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. CaYin1 NES

    Plasmid

    #33318

    Depositor

    Robert Campbell

    Article

    Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

    Insert

    CaYin1 NES
    

    Tags

    NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-mCherry-NS-MCS

    Plasmid

    #75179

    Purpose

    mCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mCherry-NS-MCS
    

    Use

    Three prime entry vector for multisite gateway th…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMZ-181

    Plasmid

    #195429

    Purpose

    Transient, constitutive expression of synZiFTR: ZF6-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF6-4 (ZF6) - p65 - RbGpA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Tag2B BimEL (3SA)

    Plasmid

    #23091

    Depositor

    Roger Davis

    Article

    Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

    Insert

    Bim EL (3SA) (Bcl2l11 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 55/65/73 to alanine

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only