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pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Article

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9 K1151TAG, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16272 Flag.Ollas.V5

Plasmid

#241490

Purpose

Flag.Ollas.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

Flag, Ollas, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16286 HA.Ollas.V5

Plasmid

#241493

Purpose

HA.Ollas.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

HA, Ollas, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16285 HA.Ollas.VSVG

Plasmid

#241504

Purpose

HA.Ollas.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

HA, Ollas, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4K16Q-Halo-FRT

Plasmid

#247458

Purpose

Expresses wild-type H4K16Q-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4K16R-Halo-FRT

Plasmid

#247457

Purpose

Expresses wild-type H4K16R-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4-HaloTag-FRT

Plasmid

#247452

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4K16A-Halo-FRT

Plasmid

#247459

Purpose

Expresses wild-type H4K16A-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

K16A

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NUP98_NSD1Δ1-746

Plasmid

#244804

Purpose

Express mEGFP-tagged fusion protein NUP98_NSD1 with an IDR deletion

Depositor

Richard Kriwacki

Article

Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi

Insert

NUP98_NSD1Δ1-746

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Deletion of an IDR (amino acids 1-746 deleted)

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FOidr_080

Plasmid

#244632

Purpose

Express mEGFP-tagged intrinsically disordered protein (IDR), FOidr_080, derived from human fusion protein KMT2A_AFDN; KMT2A_EPS15; KMT2A_MLLT1; KMT2A_MLLT10

Depositor

Richard Kriwacki

Article

Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi

Insert

FOidr_080

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Unmutated

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FOidr_018

Plasmid

#244570

Purpose

Express mEGFP-tagged intrinsically disordered protein (IDR), FOidr_018, derived from human fusion protein BCR_ABL1; BCR_JAK2

Depositor

Richard Kriwacki

Article

Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi

Insert

FOidr_018

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Unmutated

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411b

Plasmid

#246583

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 )

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-Gpr161-3xFlag

Plasmid

#245992

Purpose

Express 3xFlag C-terminal tagged mouse GPR161 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr161 (Gpr161 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Gnai3

Plasmid

#245997

Purpose

Express HA N-terminal tagged mouse Galphai3 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnai3 (Gnai3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gna15

Plasmid

#246003

Purpose

Express HA N-terminal tagged mouse Galpha15 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gna15 (Gna15 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnaz

Plasmid

#246000

Purpose

Express HA N-terminal tagged mouse Galphaz transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnaz (Gnaz Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-Gpr45-Y287C-3xFlag

Plasmid

#245977

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-Y287C mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Y287C

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-Gpr45-Del2-3xFlag

Plasmid

#245980

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-Del2 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Del2, deletion of R247-D259

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-Gpr45-Del1-3xFlag

Plasmid

#245979

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-Del1 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Del1, deletion of R223-D237

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-Gpr45-S214P-3xFlag

Plasmid

#245978

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-S214P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

S214P

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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