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pMYs-MFN2-IRES-mitoRFP

Plasmid

#121997

Purpose

Expression of MFN2 and mitoRFP in mammalial cells

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

MFN2-IRES-mitoRFP (MFN2 Human)

Use

Retroviral

Mutation

silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep128

Plasmid

#136872

Purpose

Mammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep128 (CEP128 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep78

Plasmid

#136827

Purpose

Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep78 (CEP78 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1

Plasmid

#22008

Purpose

Mammalian expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Tags

mVenus

Expression

Mammalian

Mutation

LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pWPT-/mEGFP-1T-IRES-mCherry

Plasmid

#190190

Purpose

Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Inserts

mEGFP
mCherry

Use

Lentiviral

Mutation

changed EGFP Kozak sequence inserting a C>T mo…

Promoter

EIF1-short

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_NPYmut

Plasmid

#208678

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep128

Plasmid

#136823

Purpose

Mammalian expression of the centrosomal protein Cep128 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep128 (CEP128 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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gfap-EGFP donor

Plasmid

#65564

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap-EGFP (gfap Zebrafish)

Use

Zebrafish injection

Promoter

none

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLenLox_U6 Nlgn2

Plasmid

#59358

Purpose

Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Rat, Mouse)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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CIBN-rTetR

Plasmid

#183919

Purpose

Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

CIBN-rTetR (CIB1 Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-Zfp423-deltaSBD

Plasmid

#35971

Depositor

Bruce Spiegelman

Article

Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

Insert

Zinc Finger Protein 423 (Zfp423 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

lacks zinc fingers 14-19 (aa 640 -838) (delta SMA…

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pBactin-Nedd1-mNeonGreen

Plasmid

#196864

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mNeonGreen (Nedd1 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

Plasmid

#22018

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutation T17N.

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mCerulean-PA-Rac1

Plasmid

#22031

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Cep76

Plasmid

#136813

Purpose

Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep76 (CEP76 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-SV40P-Tet2 enhancer H1

Plasmid

#63883

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 enhancer fragment H1

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-T0-Flag-HROB

Plasmid

#135298

Purpose

Expresses Flag-tagged HROB in mammalian cells

Depositor

Daniel Durocher

Article

Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

Insert

HROB (HROB Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEBTet-SNAP-Cep41

Plasmid

#136794

Purpose

Mammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep41 (CEP41 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-α-tubulin

Plasmid

#136791

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-TUBA (DNMBP Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-CIBN

Plasmid

#183937

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-CIBN (CIB1 Mustard Weed, Synthetic)

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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