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pXCX-sGFAP-LMO-IRES-tdTomato

Plasmid

#178312

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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myc-NLRC5 iso3

Plasmid

#37510

Depositor

Thomas Kufer

Article

Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

Insert

NLRC5 (NLRC5 Human)

Tags

myc

Expression

Mammalian

Mutation

isoform 3

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-Flag-mPar6a.PIG

Plasmid

#24599

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pJDC122

Plasmid

#157876

Purpose

tdTomato is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.

Depositor

Jeffrey Cirillo

Article

Kong et al PLoS One. 2016 Mar 2;11(3):e0149972. doi

Insert

tdTomato

Expression

Bacterial

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGPS21loxFRTNeo

Plasmid

#27180

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R

Use

In vitro transposion

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pGEL089

Plasmid

#137897

Purpose

iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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MSCV-Flag-mPar6aK19A

Plasmid

#24598

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19A

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20Cerulean3-Cdc8

Plasmid

#99357

Purpose

Bacterial expression of Cerulean3 fused to the 5' of cDNA of the fission yeast tropomyosin, cdc8.

Depositor

Dan Mulvihill

Article

Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

Insert

cdc8 (cdc8 Fission Yeast)

Tags

Cerulean3 and His6-Gly3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pICH87644

Plasmid

#50274

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1482

Plasmid

#50295

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + mitochondrial localisation signal ScCoxIV (Saccharomyces cerevisiae)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45195

Plasmid

#50275

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH85281

Plasmid

#50272

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, mas, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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slpr genomic fragment

Plasmid

#42074

Depositor

Norbert Perrimon

Article

Stronach et al Genes Dev. 2002 Feb 1;16(3):377-87.

Insert

slpr genomic fragment (slpr Fly)

Expression

Insect

Promoter

Hsp70

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pAGM5343

Plasmid

#50297

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (O. sativa) + HIS tag

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLXSN-p85α_wt

Plasmid

#39501

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 (PIK3R1 Bovine)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pAJ02

Plasmid

#14909

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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PA1024_pET21a(+)C-tag

Plasmid

#14579

Depositor

SeWon Suh

Article

Ha et al J Biol Chem. 2006 Jul 7. 281(27):18660-7

Insert

2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-VTKS4-GCaMP6f

Plasmid

#209931

Purpose

Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter. Based on construct from Pouchelon et al Cell Rep Methods. 2022.

Depositor

Jessica Cardin

Insert

GCaMP6f

Use

AAV, CRISPR, and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_WT

Plasmid

#224142

Purpose

IPTG-inducible expression of C. elegans protein IDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1 (F59B8.2 Nematode)

Tags

intein-CBD

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_G98N

Plasmid

#224143

Purpose

IPTG-inducible expression of C. elegans protein IDH-1(G98N) with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1(G98N)

Tags

intein-CBD

Expression

Bacterial

Mutation

G98N

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_R133H

Plasmid

#224144

Purpose

IPTG-inducible expression of C. elegans protein IDH-1(R133H) with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1(R133H)

Tags

intein-CBD

Expression

Bacterial

Mutation

R133H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEPDG1CB0003

Plasmid

#227551

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoACT3_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEPCT1CB0066

Plasmid

#227552

Purpose

Level 1 - Position 3 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoACT4_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPCT1CB0067

Plasmid

#227553

Purpose

Level 1 - Position 3 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoACT5_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPCT1CB0068

Plasmid

#227554

Purpose

Level 1 - Position 3 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoACT6_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPCT1CB0069

Plasmid

#227555

Purpose

Level 1 - Position 3 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoACT7_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPCT0CM0210

Plasmid

#227532

Purpose

L0 part - CDS (without STOP codon)

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

CoACT7 (Calendula officinalis acyltransferase 7)

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS1CB0001

Plasmid

#227533

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoTXSS_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS1CB0010

Plasmid

#227534

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CcTXSS_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS1CB0011

Plasmid

#227536

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CeTXSS_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0028

Plasmid

#227537

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_TdTXSS_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS1CB0014

Plasmid

#227538

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_LsTXSS_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS1CB0008

Plasmid

#227539

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_TkTXSS _35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS1CB0009

Plasmid

#227540

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_TcTXSS_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS1CB0003

Plasmid

#227541

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoMAS_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0004

Plasmid

#227542

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CaTXSS_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS1CB0018

Plasmid

#227543

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A392_35S

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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