We narrowed to 19,051 results for: Tec;

1. pGWB553

   Plasmid

   #74883

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   No Promoter

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB544

   Plasmid

   #74878

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_722

   Plasmid

   #216077

   Purpose

   CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Destination

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEL035

   Plasmid

   #137903

   Purpose

   STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. UPB-colon (UPB4)

   Plasmid

   #180786

   Purpose

   Unique Projection Barcode (UPB4)-Colon for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM3Dq ( partial )
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB1H1-Zif268

   Plasmid

   #12612

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

   Insert

   Zif268 in pB1H1 (Egr1 Mouse)
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. RBD#4L

   Plasmid

   #193021

   Purpose

   hnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

   Depositor

   Xue Gao

   Article

   Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

   Insert

   Cas13a
   

   Tags

   hnRNP C RRM

   Expression

   Bacterial

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEC3006 (pSpy1C-PZn_ffluc)

   Plasmid

   #218515

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PZn (PczcD)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YE2-BE4max

   Plasmid

   #138156

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YE2)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115216

    Purpose

    Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin2Cas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCH68 (dAsCas12a-3xKRAB piggyBac)

    Plasmid

    #217334

    Purpose

    piggyBac expression of dAsCas12a-3xKRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    dAsCas12a
    

    Use

    CRISPR

    Tags

    nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115208

    Purpose

    Expresses PinCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PinCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dCpf1-BE

    Plasmid

    #107685

    Purpose

    Expresses dLbCpf1-BE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE
    

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-2xFYVE_hrs

    Plasmid

    #67676

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    2xFYVEhrs
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. gRNA NIA Null

    Plasmid

    #198728

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pv6_CBX1_2xChromo

    Plasmid

    #179393

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-APOBEC1-YTHD422N

    Plasmid

    #194432

    Purpose

    APOBEC1 fused to YTHD422N

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHD422N (APOBEC1 Human)
    

    Use

    CRISPR

    Tags

    APOBEC1-YTHD422N-HA

    Expression

    Mammalian

    Mutation

    D422N mutation in the YTH domain to enhance m6A b…

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid

    #181781

    Purpose

    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Expression

    Bacterial

    Mutation

    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. dTomato_LD 0_CS M_tTA-BFP

    Plasmid

    #58875

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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