We narrowed to 14,321 results for: cas9

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pYZ300

Plasmid

#102689

Purpose

S. pombe gRNA entry vector with Cas9 - Sp his3 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ301

Plasmid

#102690

Purpose

S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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5aa SunTag VP64 CLV3

Plasmid

#115485

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

Depositor

Steven Jacobsen

Publication

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJB166

Plasmid

#86998

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection

Depositor

Julien Berro

Publication

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

Plasmid

#217802

Purpose

Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Publication

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

Plasmid

#217796

Purpose

Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Publication

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#187326

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Plasmid

#195541

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET42b-AncBE4max

Plasmid

#165157

Purpose

Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

Depositor

Taekjip Ha

Publication

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

AncBE4max

Tags

6xHis

Expression

Bacterial

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VPR

Plasmid

#79376

Purpose

Recruits VPR to compatible gRNAs for transcriptional activation

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VPR

Use

CRISPR

Expression

Mammalian

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAGM65881

Plasmid

#153215

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB3050(gRNA XII-5)

Plasmid

#73292

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB3020(gRNA X-2)

Plasmid

#73282

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJMR19-ADE2

Plasmid

#254119

Purpose

Candidozyma auris optimized vector expressing Cas9 and ADE2 targeting guide cassette

Depositor

Jeffrey Rybak

Publication

Doorley et al J Infect Dis. 2025 Sep 15;232(3):e529-e5

Insert

Candidozyma auris ADE2 target sequence

Use

CRISPR

Expression

Yeast

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pSEVA1213S

Plasmid

#122095

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX20

Plasmid

#169443

Purpose

Used for guide cloning with PCR

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Type

Empty backbone

Use

CRISPR

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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SpRYc

Plasmid

#175575

Purpose

Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Publication

Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

Insert

SpRYc

Tags

EGFP

Expression

Mammalian

Mutation

PID of SpRY grafted onto the N-terminal domain of…

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pROS10

Plasmid

#107924

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMEL16

Plasmid

#107922

Purpose

HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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