We narrowed to 14,099 results for: cas9

1. pICH47802-p35S::tdTomato-ER::tNOS

   Plasmid

   #160220

   Purpose

   tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   p2x35S::TdTomato-ER::tNOS
   

   Use

   Synthetic Biology; Moclo toolkit level 1 acceptor…

   Expression

   Plant

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPE3-pPuro

   Plasmid

   #215722

   Purpose

   Cloning vector for Uni-Vector prime editing system

   Depositor

   Kou-Juey Wu

   Article

   Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

   Insert

   PE2
   

   Use

   Unspecified

   Promoter

   EFS promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. gRNA-his-HYB

   Plasmid

   #64333

   Purpose

   his3 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of his3 deletion gRNA cassette
   

   Expression

   Yeast

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pACDura3

   Plasmid

   #138711

   Purpose

   Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Insert

   ura3 gene (URA3 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   optimized ura3 gene for expression in Escherichia…

   Promoter

   pEM7 promoter

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-TRE3G-NLS-tdMCP-mCherry

   Plasmid

   #99909

   Purpose

   For the expression of NLS-tdMCP-mCherry

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-tdMCP-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-UGCG-KO

   Plasmid

   #80010

   Purpose

   gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   UGCG (UGCG Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MASTL B4.4 gRNA

   Plasmid

   #90755

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MASTL

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MASTL (Guide Designation B4.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDuRCC-N

   Plasmid

   #81194

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please view depositor comments below and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC10

   Plasmid

   #104784

   Purpose

   pAH595-gRNA 2xplex entry cassette

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNOC-CRISPR-P2A-BlastR

    Plasmid

    #98147

    Purpose

    Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    P2A-BlastR fusion with Cas9-Nlux

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-TRE3G-NLS-tdPCP-EGFP

    Plasmid

    #99910

    Purpose

    For the expression of NLS-tPMCP-EGFP

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Insert

    NLS-tdPCP-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE4G

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AM77_pU6.Sa-gRNA.CLYBL

    Plasmid

    #199237

    Purpose

    Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. aureus gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. sg14x(MS2) MUC4.1

    Plasmid

    #101153

    Purpose

    gRNA with 14 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. gh19

    Plasmid

    #106703

    Purpose

    expression of gRNA targeting GALNT19

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    GALNT19
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 2a-copGFP(+HA)

    Plasmid

    #83806

    Purpose

    Express copGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    copGFP
    

    Use

    Homologous recombination for gapdh loci

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. KIF20A D5.2 gRNA

    Plasmid

    #90718

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF20A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF20A (Guide Designation D5.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh171

    Plasmid

    #106832

    Purpose

    expression of gRNA targeting UGCG

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGCG (UGCG Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. DKC1 F1.3 gRNA

    Plasmid

    #90652

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DKC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DKC1 (Guide Designation F1.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. DKC1 F2.3 gRNA

    Plasmid

    #90653

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DKC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DKC1 (Guide Designation F2.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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