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pRK5 Flag RagB (CTD)

Plasmid

#112769

Purpose

express Flag-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (C terminal domain only) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB (GD)

Plasmid

#112770

Purpose

express Flag-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (G domian only) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagC (GD)

Plasmid

#112767

Purpose

express GFP-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagC (G domain only) (RRAGC Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (GD)

Plasmid

#112772

Purpose

express Flag-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain only) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Flag RagC (CTD)

Plasmid

#112771

Purpose

express Flag-RagC (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (C terminal domain only) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 GFP RagB (CTD)

Plasmid

#112766

Purpose

express GFP-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (C terminal domain only) (RRAGB Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 GFP RagB (GD)

Plasmid

#112765

Purpose

express GFP-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (G domain only) (RRAGB Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX p55g(NC)-SH2

Plasmid

#46462

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p55g(NC) (PIK3R3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 56-461

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Ptk70-SH2

Plasmid

#46473

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Ptk70 (Srms Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 123-236

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Bks-SH2

Plasmid

#46406

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Bks (STAP2 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 141-266

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Slnk-SH2

Plasmid

#46502

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slnk (Sh2d6 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 199-323

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Sck-SH2

Plasmid

#46478

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Sck/ShcB (Shc2 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 464-573

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Socs7-SH2

Plasmid

#46509

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs7 (SOCS4 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 280-386

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp3-SH2

Plasmid

#46461

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp3 (SH2D3C Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 212-330

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4a (mEosFP4a-V69T)

Plasmid

#99225

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4a

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4 WW2

Plasmid

#111882

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: NEDD4 WW2 fused to C-termin…

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab32 DN

Plasmid

#37739

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T33N (dominant negative)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab32 CA

Plasmid

#37740

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q79L (constitutive active)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR V2 SQLE_sg2

Plasmid

#244872

Purpose

Knockout of human SQLE

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SQLE sgRNA (SQLE Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SQLE_sg1

Plasmid

#244871

Purpose

Knockout of human SQLE

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SQLE sgRNA (SQLE Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge