We narrowed to 150,751 results for: ins

1. pBS human IRS-1

   Plasmid

   #11359

   Depositor

   Ronald Kahn

   Article

   Araki et al Diabetes. 1993 Jul . 42(7):1041-54.

   Insert

   IRS-1 (IRS1 Human)
   

   Expression

   Bacterial

   Available Since

   March 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-ChRmine-mRuby2-ST

   Plasmid

   #227777

   Purpose

   Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChRmine-mRuby2-ST
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV500 A-C/EBP

   Plasmid

   #33352

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   C/EBP (Cebpa Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative (see notes)

   Promoter

   CMV

   Available Since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

4. CRY2olig(FL)-mCherry

   Plasmid

   #60033

   Purpose

   Expresses a fusion of full length CRY2 (E490G) with mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2 E490G (deltaNLS) (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   E490G, NLS mutated

   Promoter

   CMV

   Available Since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK2301

   Plasmid

   #65778

   Purpose

   Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   St1Cas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+) 6xHis-3xFlag-IFIT5

   Plasmid

   #53561

   Purpose

   bacterial expression of IFIT5

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT5 (IFIT5 Human)
   

   Tags

   3xFLAG, 6xHIS, and TEV site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pJRoC30_VP11H

   Plasmid

   #188063

   Purpose

   PROSS-designed versatile peroxidase 11H

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

   Insert

   PROSS-designed versatile peroxidase from Pleurotus ostreatus
   

   Tags

   S. cerevisiae native α-factor prepro-leader signa…

   Expression

   Yeast

   Mutation

   38 PROSS mutations, described in publication

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

   Plasmid

   #114106

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002

   Insert

   Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3xFlag-CMV7-Erk2_KR

   Plasmid

   #39224

   Depositor

   Melanie Cobb

   Article

   Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

   Insert

   Erk2(K52R) (Mapk1 Rat)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K52R

   Promoter

   CMV

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

10. HB501: pMVP (L1-L4) RIP

    Plasmid

    #121688

    Purpose

    pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Rat Ins1 promoter (RIP; β-cell specific)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

    Plasmid

    #79383

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Zhang 2.0 + scaffold
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AICSDP-078:G3BP1-mEGFP

    Plasmid

    #193920

    Purpose

    Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1

    Depositor

    Allen Institute for Cell Science

    Insert

    G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pFastBac1-OSF-TRIM5alpha(human)

    Plasmid

    #79034

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human) (TRIM5 Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

    Plasmid

    #200401

    Purpose

    Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Open-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVk7-33G1-Flag

    Plasmid

    #58950

    Purpose

    backbone for mouse recombinant antibody with added C-terminal FLAG tag

    Depositor

    Gavin Wright

    Article

    Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

    Type

    Empty backbone

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(+) 6xHis-TEV-IFIT1

    Plasmid

    #53557

    Purpose

    bacterial expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pET303-ColE1-T-E192C

    Plasmid

    #180233

    Purpose

    Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T domains (residue…

    Promoter

    T7

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. shCDK4/6(1)

    Plasmid

    #73554

    Purpose

    vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. MSP977

    Plasmid

    #65774

    Purpose

    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E mutation in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVk7-33G1-BioLHis

    Plasmid

    #58949

    Purpose

    backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

    Depositor

    Gavin Wright

    Article

    Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

    Type

    Empty backbone

    Tags

    6His tag and biotinylation peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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