We narrowed to 9,181 results for: mel

1. pET-NlaCascade-NLS-6xHis

   Plasmid

   #180213

   Purpose

   Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   NlaCas5-Cas8-Cas7
   

   Tags

   6XHis and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

   Plasmid

   #217510

   Purpose

   KD Recombinase dependent conditional smGdP-10XV5 cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
   

   Tags

   smGdP-10XV5

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE-puro TPR-Met

   Plasmid

   #10902

   Depositor

   Bob Weinberg

   Article

   Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

   Insert

   TPR-Met (MET, TPR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Constitutively active form of the c-Met receptor

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-shQKI-3373

   Plasmid

   #115456

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shQKI-3373 (QKI Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Puro CAG eGFP NLS

   Plasmid

   #170830

   Purpose

   Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. INCTbiosyn-pUB-HspINTBxb1

   Plasmid

   #127519

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase Bxb1.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase Bxb1 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV mKeima-LC3

   Plasmid

   #189006

   Purpose

   Fluorescent reporter for autophagy

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   mKeima, LC3 (Map1lc3b Rat)
   

   Use

   Retroviral

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIRCgp41-1

   Plasmid

   #74227

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   gp41-1 C-intein and gp41-1 N-intein

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM-Cas9HF1-recA56

   Plasmid

   #89962

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet and proD

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSECC

    Plasmid

    #60820

    Purpose

    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

    Depositor

    Tyler Jacks

    Article

    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

    Inserts

    Cas9
    Cre
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    BsmBI site eliminated by C->A; E308G

    Promoter

    EFS and EFS (after Cas9-2A)

    Available Since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. INCTbiosyn-pUB-HspINT2

    Plasmid

    #127512

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 2.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 2 coding sequence codon optimized for H. sapiens expression.
    

    Expression

    Mammalian

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. INCTbiosyn-pUB-HspINT9

    Plasmid

    #127516

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 9.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 9 coding sequence codon optimized for H. sapiens expression.
    

    Expression

    Mammalian

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. INCTbiosyn-pUB-HspINT13

    Plasmid

    #127517

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 13.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 13 coding sequence codon optimized for H. sapiens expression.
    

    Expression

    Mammalian

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVU-coHPDL WT

    Plasmid

    #174128

    Purpose

    Expresses wild type codon-optimized HPDL in mammalian cells

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX317-QKI

    Plasmid

    #115441

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    QKI (QKI Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5E--3mnx1

    Plasmid

    #74632

    Purpose

    Can be used to drive expression specifically in zebrafish motor neurons

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Promoter

    -3mnx1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEM-Cas9HF1

    Plasmid

    #89961

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9HF1
    

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. ABE8.17-m

    Plasmid

    #136298

    Purpose

    expresses ABE8.17-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.17-m
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. GAL-p300-230-670

    Plasmid

    #89099

    Purpose

    mammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 230-670

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GAL-p300-1601-1900

    Plasmid

    #89102

    Purpose

    mammalian expression of human p300 amino acids 1601-1900 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 1601-1900

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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