We narrowed to 9,339 results for: mel

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p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Article

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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KHBD00216

Plasmid

#34365

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG15509 (kay Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pGTM_SUMO_PLpro_W106F_001

Plasmid

#234491

Purpose

Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a W106F mutation to allow for 5-fluorotryptophan incorporation and 19F NMR

Depositor

Gaetano Montelione

Article

De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do

Insert

PLpro (ORF1ab SARS-CoV-2)

Tags

6xHis-Sumo

Expression

Bacterial

Promoter

T7 ac

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.IRES.dTomato

Plasmid

#187191

Purpose

Lentiviral gene overexpression (backbone/control)

Depositor

Jan-Henning Klusmann

Article

Gialesaki et al Blood. 2023 Mar 9;141(10):1105-1118. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-MYO10-BioID

Plasmid

#145137

Purpose

Expresses EGFP-MYO10-BioID construct in mammalian cells

Depositor

Johanna Ivaska

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

MYO10-BioID (MYO10 Human)

Expression

Mammalian

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1006

Plasmid

#235724

Purpose

Expression of FL human Beclin 1 in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Insert

Beclin 1 (BECN1 Human)

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3342 PB-PEmax-puro

Plasmid

#226674

Purpose

PiggyBac cargo vector encoding PEmax and puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

PEmax

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMTHFD1

Plasmid

#217436

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting MTHFD1 (MTHFD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-TK2-3xFLAG

Plasmid

#217427

Purpose

Lentiviral overexpression of human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

TK2 (TK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_4

Plasmid

#217431

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 4 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_5

Plasmid

#217432

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 5 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgSHMT2

Plasmid

#217435

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting SHMT2 (SHMT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3328 PB-sfGFP-WPRE-target

Plasmid

#226676

Purpose

PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

sfGFP-WPRE-site3target

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only
pTBL3428 MTK234-LacO-BtoApegRNA

Plasmid

#226669

Purpose

MTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNA

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

hU6-LacOS-BtoApegRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-LacOS

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2670 MTK3-PEmax

Plasmid

#226663

Purpose

MTK3 part containing the coding sequence of PEmax

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

PEmax

Use

CRISPR and Synthetic Biology

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3401 BtoApegRNA-sfGFP

Plasmid

#226667

Purpose

PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

BtoA pegRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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