162,050 results

1. lentiCRISPRv2FE-ABE8e-SpRY

   Plasmid

   #213008

   Purpose

   A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-HDR-mEGFP-Actin

   Plasmid

   #119870

   Purpose

   AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   beta Actin (Actb Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

   Plasmid

   #221359

   Purpose

   Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV5)

   Viral Prep

   #65014-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. hSyn-driven Jaws-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Promoter

   Syn

   Tags

   GFP

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pET28c-F30-Broccoli

   Plasmid

   #66788

   Purpose

   Expresses Broccoli in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

   Insert

   Broccoli in the F30 scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-PITCh-LMNB1

   Plasmid

   #207770

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBSTNAV

   Plasmid

   #45801

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC70364

   Plasmid

   #209515

   Purpose

   Cloning in complete guide expression cassettes, with Cas9

   Depositor

   Cristobal Uauy, Wendy Harwood

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC6A15

   Plasmid

   #131877

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A15 (SLC6A15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid

    #180484

    Purpose

    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

    Depositor

    Julie Welburn

    Article

    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

    Insert

    CENP-E (CENPE Synthetic, human, Human)
    

    Use

    Tags

    mNeon-6xHis

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_479

    Plasmid

    #179099

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZac2.1 gfaABC1D-cyto-GCaMP8s

    Plasmid

    #186052

    Purpose

    AAV-mediated expression of GCaMP8s under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    dSV40 Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. tetO.Nfib.Hygro

    Plasmid

    #117271

    Purpose

    Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Nfib (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    IRES and hygromycin resistant cassette (C termina…

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH6-MBP-TEV-BirA

    Plasmid

    #179694

    Purpose

    Expresses His6 tagged MBP-BirA fusion protein

    Depositor

    Mark Arbing

    Insert

    BirA
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Multiplex CRISPR/Cas9 Assembly System Kit

    Plasmid Kit

    #1000000055

    Purpose

    Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack

    Depositor

    Takashi Yamamoto

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Ddo_porcupine-1

    Plasmid

    #107631

    Purpose

    In situ hybridization and RNAi studies in planarians

    Depositor

    Alejandro Sánchez

    Article

    Accorsi et al The American Biology Teacher 79(3):208-2

    Insert

    porcupine-1
    

    Use

    RNAi; In situ hybridization

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid

    #218874

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    Synapsin

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. LV-GFP

    Plasmid

    #25999

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-GFP (H2BC21 Human, A. victoria)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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