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mEos2-Integrin-Beta3-N-18

Plasmid

#57391

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Beta3

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pIIIA/MS2-2

Plasmid

#220632

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pIII/MS2-1

Plasmid

#220629

Purpose

Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pIII/MS2-2

Plasmid

#220630

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pXP418

Plasmid

#26843

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pVH21-Tier1-PhEF1a

Plasmid

#169561

Purpose

Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDest-663 (attR4-attR2)

Plasmid

#161884

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Expression

Bacterial and Yeast

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXD017 KIKO AAV vector

Plasmid

#192152

Purpose

KIKO AAV vector

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAH121

Plasmid

#164901

Purpose

The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.

Depositor

Keith Shearwin

Article

Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

Insert

phi21 Integrase

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pECO200

Plasmid

#169765

Purpose

Express SpCas9 and Basta resistance gene

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Plant binary vector

Expression

Plant

Promoter

35S, AtU6

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AC

Plasmid

#108686

Purpose

Plasmid for assembly of donor DNA templates consisting of two expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AD

Plasmid

#108687

Purpose

Plasmid for assembly of donor DNA templates consisting of three expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Ftz

Plasmid

#11243

Depositor

Robin Reed

Article

Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

Ftz (ftz Fly)

Expression

Bacterial

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pICH41308::hCas9

Plasmid

#49770

Purpose

Level 0 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

hCas9

Use

CRISPR

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCoofy60

Plasmid

#122012

Purpose

Baculovirus vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

Depositor

Peggy Stolt-Bergner, Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-eGFP

Expression

Insect

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTYB2-eIF4B

Plasmid

#37226

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF3

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pECO300

Plasmid

#169766

Purpose

Express SpCas9 and Kanamycin resistance gene

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Plant binary vector

Expression

Plant

Promoter

35S, AtU6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCR8/GW:U6

Plasmid

#85981

Purpose

Golden Gate Level 0 cassette encoding the U6 promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

T. pseudonana U6 promoter

Use

CRISPR; Diatom (t. pseudonana)

Promoter

none

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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EC10161

Plasmid

#154060

Purpose

Level 0.5 Golden Gate backbone, containing loxP sites.

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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