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pLG008 (Ec78 retron)

Plasmid

#157886

Purpose

Expresses the Ec78 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec78 retron

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proD-B0032-E0051

Plasmid

#107241

Purpose

Expresses lacZa-GFP fusion from constitutive proD promoter

Depositor

Joseph Davis, Robert Sauer

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Promoter

proD

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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DGK gamma

Plasmid

#35408

Depositor

Robert Lefkowitz, Stephen Prescott

Article

Nelson et al Science. 2007 Feb 2;315(5812):663-6.

Insert

DGK gamma (DGKG Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pSELIS-RamR

Plasmid

#202631

Purpose

Enables dual growth and fluorescence based enrichment of RamR biosensors

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

Sh ble

Use

Synthetic Biology

Expression

Bacterial

Promoter

Lambda promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-HIS-Tol2

Plasmid

#112486

Purpose

functional recombinant Tol2 protein from E.coli

Depositor

Stephen Ekker

Article

Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

Insert

Tol2 Transposase (tpase Synthetic)

Tags

HIS/6x

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pKG1334_pHarbor-PiggyBac_backbone

Plasmid

#239706

Purpose

PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated int

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLG010 (DRT type 2)

Plasmid

#157888

Purpose

Expresses the DRT type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 2

Expression

Bacterial

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAK02

Plasmid

#19001

Depositor

Jay Keasling

Article

Khlebnikov et al J Bacteriol. 2000 Dec . 182(24):7029-34.

Insert

arabinose transporter araE under control of Ptac (araE E. coli)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
pGTQL1211YN

Plasmid

#61704

Purpose

Gateway compatible BiFC vector (pEarleyGate201-YN)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-N and HA

Expression

Plant

Promoter

35S

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGTQL1221YC

Plasmid

#61705

Purpose

Gateway compatible BiFC vector (pEarleyGate202-YC)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-C and FLAG

Expression

Plant

Promoter

35S

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGL2_163

Plasmid

#199095

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pWT PolI

Plasmid

#11721

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I (polA E. coli)

Expression

Bacterial

Mutation

wild-type

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pPTK002-2-pGAP

Plasmid

#84961

Purpose

Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glyceraldehyde-3-phosphate dehydrogenase promoter

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1805 c to g)

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK003-2-pENO1

Plasmid

#84962

Purpose

Type 2, pENO1, Enolase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Enolase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (2350 g to c) and BsmBI site re…

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::traI

Plasmid

#189575

Purpose

Tra synthase (3OC8-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-BBa_B0015

Plasmid

#72998

Purpose

BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

BBa_B0015 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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prCAG-BoxB

Plasmid

#191122

Purpose

Transcription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Inserts

rCAG
BoxB

Use

Synthetic Biology

Promoter

tetracycline-inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCF1135

Plasmid

#222106

Purpose

E. coli - Veillonella shuttle plasmid with single selectable marker

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Type

Empty backbone

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Available Since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPS-hSox2-gfp

Plasmid

#49390

Purpose

mammalian expression of human Sox2 fused to EGFP

Depositor

Randall McKinnon

Insert

Sox2 (SOX2 Human)

Use

Cre/Lox; Acceptor vector

Tags

EGFP

Expression

Mammalian

Mutation

TGA (stop) to CCA (Pro) at nt 1027 with respect t…

Promoter

CMV

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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