We narrowed to 64,621 results for: cell;

1. pSLIK-IDH2-R172K-FLAG

   Plasmid

   #66807

   Purpose

   Tet-inducible expression of mutant IDH2 in mammalian cells

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   IDH2 (IDH2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW TetO HA-AMPKa1 WT

   Plasmid

   #69824

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVAX1-cGAS

   Plasmid

   #196624

   Purpose

   Express mouse cGAS protein in mammalian cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   cGAS (Cgas Mouse)
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.2/V5-DEST CDC42EP1 WT

   Plasmid

   #69819

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.2/V5-DEST CDC42EP1 S192A

   Plasmid

   #69820

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S192A

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD40-His/V5-c-Myc

   Plasmid

   #45597

   Depositor

   Rosalie Sears

   Article

   Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

   Insert

   c-Myc (Myc Mouse)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-hPhyBY276H-mCherry-HRasCT

   Plasmid

   #100540

   Purpose

   Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
   

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-908 aa), Y276H muta…

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIZ-EGFP-MYO10-HMM

   Plasmid

   #87257

   Purpose

   Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Expression

   Insect

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   OpIE2

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tom20-CR

   Plasmid

   #171461

   Purpose

   Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

   Depositor

   Takafumi Miyamoto

   Article

   Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

   Insert

   Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-Rab8a-WT

    Plasmid

    #101048

    Purpose

    Expresses HA-tagged human Rab8-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-Rab8a-CA (Q67L)

    Plasmid

    #101050

    Purpose

    Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINDUCER-21-HOXA10

    Plasmid

    #97042

    Purpose

    Expresses HOXA10 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Homeobox A10 (HOXA10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pTT3-SP-6xHis-KIR2DL5(FL)-FLAG

    Plasmid

    #157623

    Purpose

    Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL5 (KIR2DL5A Human)
    

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD649-HAsp-mCherry-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156581

    Purpose

    Mammalian expression of mCherry fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    mCherry
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

    Plasmid

    #201120

    Purpose

    RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    TurboRFP; H2B-RFP
    

    Use

    Lentiviral

    Mutation

    No

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT2-HA-RAPGEF2(S1244A/S1248A)

    Plasmid

    #110163

    Purpose

    Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

    Insert

    RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S1244A/S1248A

    Promoter

    CMV

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER-21-RUNX1-P2A-ERG

    Plasmid

    #97045

    Purpose

    Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1, ETS-Related Gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pINDUCER-21-HOXA9

    Plasmid

    #97041

    Purpose

    Expresses HOXA9 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    HOXA9 (HOXA9 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Donor_ANKRD1_KO_Puro

    Plasmid

    #186667

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    ANKRD1 KO Puromycin (ANKRD1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Donor_YTHDF2_KO_Hygro

    Plasmid

    #186671

    Purpose

    Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Hygromycin (YTHDF2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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