We narrowed to 147 results for: B SUBTILIS¨
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Plasmid Kit#1000000115PurposeA collection of Bacillus subtilis strains in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.DepositorApplicationStrain CollectionsVector TypeBacterial ExpressionAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only
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pIA028
Plasmid#200419PurposethrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloningDepositorTypeEmpty backboneTagsPAmCherry1ExpressionBacterialAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIA033
Plasmid#200420PurposethrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.DepositorTypeEmpty backboneTagsbsPAmCherryExpressionBacterialAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKV67
Plasmid#254865Purposetetracycline induction system for B. subtilis (shuttle vector)DepositorInsertstetracycline repressor
mcherry
spectinomycin adenyltransferase
erythromycin ribosomal methylase
beta-lactamase
ExpressionBacterialAvailable SinceApril 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
pIA034
Plasmid#200421PurposeamyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.DepositorTypeEmpty backboneTagsbsdronPAExpressionBacterialAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIA030
Plasmid#200424PurposethrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorInsertLacI
TagsPAmCherryExpressionBacterialPromoterPpenAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG166
Plasmid#124366PurposeGfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilisDepositorAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG163
Plasmid#124365PurposeIPTG inducible system (gfpmut2 with Ppsank) for expression in B. subtilisDepositorAvailable SinceJuly 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA035
Plasmid#200426PurposeamyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorInsertLacI
TagsdronPAExpressionBacterialPromoterPpenAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG169
Plasmid#124367PurposeXylose inducible system with the CRE site for expression in B. subtilisDepositorAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG320
Plasmid#124370PurposeVanillic acid inducible system for expression in B. subtilisDepositorInsertgfpmut2
ExpressionBacterialPromoterPspank(V)Available SinceJan. 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pIA036
Plasmid#200425PurposeamyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorInsertLacI
TagsbsdronPAExpressionBacterialPromoterPpenAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS1C
Plasmid#55168PurposeEmpty vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxAvailable SinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLG231
Plasmid#124369PurposeIPTG inducible system (gfpmut2 with Ppsank and riboJ) for expression in B. subtilisDepositorInsertgfpmut2
ExpressionBacterialPromoterPspankAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pProUSER13F4C
Plasmid#178023PurposeE. coli - B. subtilis nicking vector pProUSER13F4C. Ap-BBR1; ΔsigF-insertion-site; Km; manR-PmanADepositorInsertnicking site to be replaced with your gene of interest
ExpressionBacterialPromoterPmanAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pProUSER13E3F
Plasmid#178026PurposeE. coli - B. subtilis nicking vector pProUSER13E3F. Ap-BBR1; yhgE-insertion-site; Spc; tetR-PtetDepositorInsertnicking site to be replaced with your gene of interest
ExpressionBacterialPromoterPtetAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pProUSER13G2F
Plasmid#178027PurposeE. coli - B. subtilis nicking vector pProUSER13G2F . Ap-BBR1; rsbP-insertion-site; Ery; tetR-PtetDepositorInsertnicking site to be replaced with your gene of interest
ExpressionBacterialPromoterPtetAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyExpressionBacterialPromoterPveg promoter with CuO sequence and PxylR promoterAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAimR
Plasmid#98687PurposeExpresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tagDepositorInsertAimR
TagsHis-tagExpressionBacterialPromoterNativeAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJAB988
Plasmid#133920PurposeminiICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoterDepositorInsertminiICE-tet(M)cat(PC194)_PT7-GFP
UseSynthetic BiologyExpressionBacterialPromoterT7 promoterAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
SMT503 - S77A
Plasmid#226842PurposePlasmid that encodes a B. Subtilis lipase (CLIP-BsLipA-eGFP) gene fusion with the S77A catalytic mutant. Compatible with PURExpress for in vitro transcription/translation. ChlorampDepositorInsertLipase A
UseSynthetic BiologyTagsSNAP and eGFPExpressionBacterialMutationR33Q, D34N, K35D, S77A, K112D, M134D, Y139C, I157MPromoterT7Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVR
Plasmid#84718PurposeE. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilisDepositorInsertsE. coli RecA promoter
5' UTR of queC gene
ExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA5a-sufCDSUB
Plasmid#195055PurposeExpresses SUF operon from B. subtilis from IPTG-inducible promoterDepositorInsertsufCDSUB
ExpressionBacterialAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
SMT503
Plasmid#226840PurposePlasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. ChlorampDepositorInsertLipase A
UseSynthetic BiologyTagsSNAP and eGFPExpressionBacterialMutationR33Q, D34N, K35D, K112D, M134D, Y139C, I157MPromoterT7Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJMP1273
Plasmid#119265PurposeE. coli attTn7 site with B. subtilis flanking sequence cloned into pACYCDepositorInsertE. coli attTn7
ExpressionBacterialAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03
Plasmid#28003DepositorInsertAT1.03
TagsmVenus and mseCFPExpressionYeastAvailable SinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only