We narrowed to 1,931 results for: cas9 expression vector

1. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGW_RUBY_EggCas9

   Plasmid

   #225990

   Purpose

   To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDual_dsCas9_Venus

   Plasmid

   #214678

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDual_dsCas9_BFP

   Plasmid

   #214679

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDual_dsCas9_Blast

   Plasmid

   #214681

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDual_dsCas9_Puro

   Plasmid

   #214680

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122089

   Purpose

   U6 driven SpCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. NmCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122091

   Purpose

   U6 driven NmCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC-6-AtSpCas9-9

   Plasmid

   #197778

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   AtSpCas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9-EcRT-Z3-Hyg

    Plasmid

    #232095

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9-EcRT-Z3-Nat

    Plasmid

    #232094

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-3×RFP-P2A-CASANOVA/gRNA telo

    Plasmid

    #137711

    Purpose

    Vector co-expressing dCas9-3×RFP-P2A-CASANOVA and sgRNA telo

    Depositor

    Dominik Niopek

    Insert

    dCas9-3×RFP-P2A-CASANOVA/gRNA telo
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo

    Plasmid

    #137712

    Purpose

    Vector co-expressing dCas9-3×RFP-P2A-wtAcrIIA4 and sgRNA telo

    Depositor

    Dominik Niopek

    Insert

    dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112924

    Purpose

    piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Piggybac transposon vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112922

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

    Plasmid

    #112921

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-hCas9D10A

    Plasmid

    #52101

    Purpose

    The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter

    Depositor

    Izuho Hatada

    Insert

    hCas9D10A
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pENTR-Cas9-TagBFP

    Plasmid

    #236725

    Purpose

    Cloning vector Cas9-TagBFP

    Depositor

    David Bersten

    Insert

    Cas9-TagBFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDe-CAS9

    Plasmid

    #61433

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUBI4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCas9-EcRT-Z3-Kan

    Plasmid

    #232096

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196079

    Purpose

    Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196081

    Purpose

    Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196083

    Purpose

    Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196075

    Purpose

    Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196077

    Purpose

    Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDSAY-vasaCas9sv40

    Plasmid

    #173669

    Purpose

    Cas9 expression vector for Anopheles

    Depositor

    Eric Marois

    Insert

    Cas9 under control of vasa promoter and SV40 terminator
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid

    #214690

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge