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We narrowed to 1,068 results for: golden gate assembly

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Showing: 101 - 150 of 1068 results

  1. pOPS0253

    Plasmid
    #115505
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115505-plasmid-map-sequence-id-224288

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  9. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  20. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  21. L0I75_tUBQ10_GG5-GG6

    Plasmid
    #219714
    Purpose
    Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG5-tUBQ10-GG6
    Use
    Synthetic Biology
    Mutation
    WT terminator with added golden gate linkers
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219714-plasmid-map-sequence-id-439236

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  22. L0I89-pMUTE_GG0-1

    Plasmid
    #219708
    Purpose
    Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pMUTE-GG1
    Use
    Synthetic Biology
    Mutation
    WT promoter with added golden gate linkers
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    219708-plasmid-map-sequence-id-435737

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  23. L0I74-_tUBQ10_GG4-GG6

    Plasmid
    #219713
    Purpose
    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-tUBQ10-GG6
    Use
    Synthetic Biology
    Mutation
    WT terminator with added golden gate linkers
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219713-plasmid-map-sequence-id-435864

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  24. L0I87-SPCHPromoter_GG0-1

    Plasmid
    #219707
    Purpose
    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH-GG1
    Use
    Synthetic Biology
    Mutation
    WT promoter with added golden gate linkers
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219707-plasmid-map-sequence-id-435861

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  25. L0I84 - GG0-pAHP6-GG1

    Plasmid
    #219706
    Purpose
    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6-GG1
    Use
    Synthetic Biology
    Mutation
    WT promoter with added golden gate linkers
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219706-plasmid-map-sequence-id-435736

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  28. pOGG014

    Plasmid
    #113988
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 4
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113988-plasmid-map-sequence-id-223089

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  29. pOGG015

    Plasmid
    #113989
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 5
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113989-plasmid-map-sequence-id-223087

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  30. pOGG013

    Plasmid
    #113987
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 3
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113987-plasmid-map-sequence-id-223414

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  31. pOGG016

    Plasmid
    #113990
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 6
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113990-plasmid-map-sequence-id-223084

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  32. pOGG203

    Plasmid
    #115504
    Purpose
    Clone of a level 1 unit which encodes a constitutively-expressed mCherry
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    pL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    115504-plasmid-map-sequence-id-223070

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  33. pKT3Ts-goldyTALEN

    Plasmid
    #80330
    Purpose
    Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence
    Depositor
    Jeffrey Essner
    Article
    Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi
    Type
    Empty backbone
    Use
    TALEN
    Expression
    Bacterial
    Promoter
    T3
    Available Since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80330-plasmid-map-sequence-id-156328

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  38. GGE114

    Plasmid
    #120731
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolytica
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Type
    Empty backbone
    Use
    Cloning/assembly plasmid
    Available Since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120731-plasmid-map-sequence-id-259124

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  39. GGE038

    Plasmid
    #120785
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_Zeta-NotI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120785-plasmid-map-sequence-id-263183

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  40. GGE067

    Plasmid
    #120784
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_Zeta-NotI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120784-plasmid-map-sequence-id-263182

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  41. GGE252

    Plasmid
    #120791
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_GSY-NotI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120791-plasmid-map-sequence-id-263208

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  42. GGE253

    Plasmid
    #120790
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_GSY-NotI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120790-plasmid-map-sequence-id-263205

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  43. GGE209

    Plasmid
    #120793
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp-Mfe
    Use
    Cloning/assembly plasmid
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120793-plasmid-map-sequence-id-263228

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  44. GGE094

    Plasmid
    #120787
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_Zeta-SfiI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120787-plasmid-map-sequence-id-263185

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  45. GGE091

    Plasmid
    #120786
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_Zeta-SfiI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120786-plasmid-map-sequence-id-263184

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  46. GGE254

    Plasmid
    #120789
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_Lip-NotI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120789-plasmid-map-sequence-id-263222

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  47. GGE255

    Plasmid
    #120788
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_Lip-NotI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120788-plasmid-map-sequence-id-310818

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  50. GGE207

    Plasmid
    #120792
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown-Mfe
    Use
    Cloning/assembly plasmid
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120792-plasmid-map-sequence-id-263227

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Showing: 101 - 150 of 1068 results