We narrowed to 1,236 results for: POLI

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pCMV6-AN-DDK_1-420 POLI

Plasmid

#131234

Purpose

Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Poliovirus 1 PV1 isolate 09_05_19 3C protease

Plasmid

#228647

Purpose

Bacterial expression of the Polio 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Polio virus 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pspCas9-POLI-ST2-com vector

Plasmid

#176234

Purpose

Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI5M

Plasmid

#249060

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(H840A)-PolI5M

Plasmid

#249064

Purpose

Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9(H840A)-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pOT2-poly-cis

Plasmid

#141177

Purpose

To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter

Depositor

Guiliang Tang

Type

Empty backbone

Use

RNAi and Synthetic Biology

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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HO-Poly-HO

Plasmid

#51661

Purpose

yeast plasmid for integration of target sequence at HO locus

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)

Plasmid

#65524

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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P63-sp64 Poly A

Plasmid

#59435

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 63

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
P29-sp64 Poly A

Plasmid

#59434

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 29

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
P21-sp64 Poly A

Plasmid

#59433

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 21

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
poly H35S SUMO1

Plasmid

#221071

Purpose

Expression of poly H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35S

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly E67R SUMO1

Plasmid

#221072

Purpose

Expression of poly E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

E67R

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35K SUMO1

Plasmid

#221070

Purpose

Expression of poly H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35K

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35R SUMO1

Plasmid

#221069

Purpose

Expression of poly H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35R

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBAC3-POLY-pnRFP

Plasmid

#201680

Purpose

Boost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

813

Expression

Mammalian

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-POLY-eRF1(E55D)

Plasmid

#157925

Purpose

Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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HO-Poly-KanMX4-HO

Plasmid

#51662

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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