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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 893 results for: Px330

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Showing: 41 - 60 of 893 results

  1. pdCas9-Tet1-CD

    Plasmid
    #83340
    Purpose
    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system
    Depositor
    Ronggui Hu
    Publication
    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    U6, CBH
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    83340-plasmid-map-sequence-id-334608

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  6. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

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  7. pX330-sgR26

    Plasmid
    #127376
    Purpose
    Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.
    Depositor
    Lance Miller
    Publication
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ROSA26 (Gt(ROSA)26Sor Mouse)
    Use
    CRISPR and Mouse Targeting
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    127376-plasmid-map-sequence-id-248005

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  14. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Publication
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  16. pX330 _CRISPIE donor B7

    Plasmid
    #172848
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172848-plasmid-map-sequence-id-341024

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Showing: 41 - 60 of 893 results