Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,070 results for: gem

Clear keywords
Showing: 41 - 60 of 2070 results

  3. GEM

    Plasmid
    #38820
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GEM (Gem Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pAN1232

    Plasmid
    #127164
    Purpose
    Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)
    Depositor
    Hana El-Samad
    Publication
    Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
    Use
    Synthetic Biology
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    127164-plasmid-map-sequence-id-250325

    Enlarge

  7. pGEMS-II

    Plasmid
    #204765
    Purpose
    GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-m6Alinker-DHFR
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204765-plasmid-map-sequence-id-408664

    Enlarge

  8. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

    Enlarge

  12. pGEMS-II-dCas13-APO1-YTH

    Plasmid
    #204767
    Purpose
    GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-NLS-APO1-YTH-HA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    Ef1alpha
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204767-plasmid-map-sequence-id-408667

    Enlarge

  14. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

    Plasmid
    #190181
    Purpose
    Fluorescent reporter vector to visualize all of cell cycle phases.
    Depositor
    Toshiro Sato
    Publication
    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    TagBFP2
    hGeminin(1/110)
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    190181-plasmid-map-sequence-id-378849

    Enlarge

  15. pHES839

    Plasmid
    #87941
    Purpose
    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.
    Depositor
    Hana El-Samad
    Publication
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Human, Synthetic)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    ADH1 (crippled)
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87941-plasmid-map-sequence-id-180360

    Enlarge

  16. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

    Plasmid
    #169338
    Purpose
    Expression of the LT-Fucci(CA) in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fucci(CA) (GMNN, CDT1 Human)
    Tags
    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…
    Expression
    Mammalian
    Mutation
    HaloTag9 = HaloTag7-Q165H-P174R
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169338-plasmid-map-sequence-id-333246

    Enlarge

  17. pcDNA3.1+ GEMIN6 myc HIS

    Plasmid
    #71659
    Purpose
    Mammalian expression of GEMIN6
    Depositor
    Gideon Dreyfuss
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEMIN6 (GEMIN6 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

    Plasmid
    #169337
    Purpose
    Expression of the LT-Fucci(SCA) in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fucci(SCA) (GMNN, CDT1 Human)
    Tags
    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…
    Expression
    Mammalian
    Mutation
    HaloTag9 = HaloTag7-Q165H-P174R
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    169337-plasmid-map-sequence-id-333245

    Enlarge

  19. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

    Plasmid
    #169336
    Purpose
    Expression of the LT-Fucci(SA) in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fucci(SA) (GMNN, CDT1 Human)
    Tags
    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)
    Expression
    Mammalian
    Mutation
    HaloTag9 = HaloTag7-Q165H-P174R
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    169336-plasmid-map-sequence-id-333244

    Enlarge

Showing: 41 - 60 of 2070 results