We narrowed to 3,469 results for: gem
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Plasmid#183475PurposeThis plasmid can be used to prepare EGFP mRNA by In Vitro TranscriptionDepositorInsertEGFP
ExpressionBacterialPromoterT7Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediatePromoterT7Available SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMEX174-hCALML3
Plasmid#47600DepositorAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/601
Plasmid#26656DepositorInsert
ExpressionBacterialAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorInsertEGFP-PEST-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Cas9-hGem_gRNA2
Plasmid#217659Purposeexpresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locusDepositorInsertsgRNA2 targeting the human AAVS1 safe harbour locus
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
ExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-Nanog
Plasmid#16351DepositorInsertHomo sapiens Nanog homeobox (NANOG Human)
ExpressionBacterialAvailable SinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEMTEZ-TeTxLC
Plasmid#32640DepositorInsertTetanus toxin light chain
ExpressionBacterialAvailable SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pGEM-HSPA5P
Plasmid#44150Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)DepositorInsertHSPA5 promoter (HSPA5 Human)
ExpressionMammalianAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-Fluc
Plasmid#183474PurposeThis plasmid can be used to prepare Fluc mRNA by In Vitro TranscriptionDepositorInsertFirefly luciferase
UseLuciferaseExpressionBacterialPromoterT7Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCas9-Geminin
Plasmid#109401PurposeExpresses Cas9 fusion to Geminin degron domainDepositorInsertGeminin (GMNN Human)
MutationGeminin degron fragmentAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-hGeminin
Plasmid#199344Purposemodified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human GemininDepositorInsertATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
Tagscas9 c-term fused to hgemenin 1-110 fragmentExpressionMammalianMutationcas9 c-term fused to hgemenin 1-110 fragmentPromotercbhAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM-LIN28
Plasmid#16350DepositorInsertHomo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
ExpressionBacterialAvailable SinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-h_bPAC_cmyc
Plasmid#28134Purposeoptogenetic tool for light modulation of cellular cAMPDepositorInserthumanized photoactivated adenylyl cyclase
UseIn vitro transcriptionTagsMycAvailable SinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM2 hTSP-1
Plasmid#12993DepositorInsertTSP-1 (THBS1 Human)
ExpressionBacterialAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEM_H3.2-HaloTag
Plasmid#244240PurposeCRISPR/Cas9 donor plasmid to tag human histone H3.2 with Halo tagDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-OMA1
Plasmid#61718Purposehuman Oma1 protease in the E.coli/in vitro translation vectorDepositorInsertOMA1 (OMA1 Human)
ExpressionBacterialAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only