We narrowed to 2,067 results for: gem
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Plasmid#32463DepositorInsertGEM-GECO1
UseLab constructedTags6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…Available sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GEM
Plasmid#38820PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Geminin
Plasmid#12174DepositorPublicationInsertGeminin (gmnn Frog)
ExpressionMammalianAvailable sinceJuly 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAN1232
Plasmid#127164PurposeVector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)DepositorInsertpRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
UseSynthetic BiologyAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV mAG h-Geminin
Plasmid#206281PurposeENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMVDepositorAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorInsertEGFP-PEST-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediatePromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Clover-Geminin(1-110)
Plasmid#83915PurposeFluorescent probe for M/G1 transitionDepositorInsertClover-Geminin(1-110)
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyExpressionMammalianPromoterEf1alphaAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21
Plasmid#105519PurposeT7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNADepositorAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64
Plasmid#203348PurposeTemplate for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertEnhanced Green Fluorescent Protein
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …PromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHES839
Plasmid#87941PurposeConstitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
UseSynthetic BiologyExpressionYeastPromoterADH1 (crippled)Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2
Plasmid#190181PurposeFluorescent reporter vector to visualize all of cell cycle phases.DepositorInsertsExpressionBacterial and MammalianPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-
Plasmid#169338PurposeExpression of the LT-Fucci(CA) in mammalian cellsDepositorTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1+ GEMIN6 myc HIS
Plasmid#71659PurposeMammalian expression of GEMIN6DepositorAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+
Plasmid#169337PurposeExpression of the LT-Fucci(SCA) in mammalian cellsDepositorTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)
Plasmid#169336PurposeExpression of the LT-Fucci(SA) in mammalian cellsDepositorTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PfV-Sapphire-IRES-DSRed
Plasmid#116934PurposeMammalian lenti vector for expression of the 40nm GEM and DSRedDepositorInsertPfv
UseLentiviralTagsSapphirePromoterCMVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by