We narrowed to 14,622 results for: nts

1. VinculinTS-A50I/I997A

   Plasmid

   #111829

   Purpose

   Vinculin tension sensor with point mutations affecting ability to bind talin and actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Expression

   Mammalian

   Mutation

   changed vinculin alanine 50 to isoleucine and iso…

   Promoter

   CMV

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL-VinculinTS-A50I

   Plasmid

   #111831

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (A50I) (VCL Chicken)
   

   Use

   Lentiviral

   Mutation

   changed vinculin alanine 50 to isoleucine

   Promoter

   CMV

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. VinTS-tCRMod-GGSGGS5

   Plasmid

   #111763

   Purpose

   Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGL4.11-COL1A1double mutants

   Plasmid

   #230918

   Purpose

   Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.

   Depositor

   Avi Rosenberg

   Article

   Yang et al Lab Invest. 2024 May;104(5):100336. doi:

   Insert

   Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase-luc2p

   Expression

   Bacterial and Mammalian

   Mutation

   COL1A1 promoter with mutant SMAD and TEAD binding…

   Promoter

   COL1A1 with mutant SMAD and TEAD elements

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-VcnTS-E1015A

   Plasmid

   #213415

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1015A (VCL Chicken, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-VcnTS-E1021A

   Plasmid

   #213416

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1021A (VCL Chicken, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL-VinculinTS-S1033A

   Plasmid

   #211835

   Purpose

   Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinTS-S1033A (VCL Chicken, Synthetic)
   

   Use

   Lentiviral

   Mutation

   mutated vinculin serine 1033 to alanine (S1033A),…

   Promoter

   CMV

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL-VinculinTS-S1033D

   Plasmid

   #211893

   Purpose

   Vinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinTS-S1033D (VCL Chicken, Synthetic)
   

   Use

   Lentiviral

   Mutation

   mutated vinculin serine 1033 to aspartic acid (S1…

   Promoter

   CMV

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_003 sgINTS12 guide 2

   Plasmid

   #193607

   Purpose

   INTS12 knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgINTS12 guide 2 (INTS12 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_003 sgINTS12 guide 1

    Plasmid

    #193606

    Purpose

    INTS12 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgINTS12 guide 1 (INTS12 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-MNTS-1-p75

    Plasmid

    #182945

    Purpose

    To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTR

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

    Insert

    MNTS-1-p75 (NTF3, NT3 Human, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV promoter and enhancer

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2 NtSPrbcS (GB2213)

    Plasmid

    #160589

    Purpose

    B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain

    Depositor

    Diego Orzaez

    Insert

    NtSPrbcS
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSUI-SynP-CANTsg

    Plasmid

    #154343

    Purpose

    Expression of the sgRNA targeting to the E. coli can gene

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Insert

    CANT sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTOPO-rDNA-NTS

    Plasmid

    #23992

    Depositor

    Susan Baserga

    Article

    Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

    Insert

    rDNA NTS
    

    Use

    Bacterial cloning - topo

    Mutation

    None

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pUb FLAG-Human IntS6

    Plasmid

    #198408

    Purpose

    Expresses FLAG-tagged human IntS6

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    DDX26 (INTS6 Human)
    

    Tags

    3x FLAG

    Expression

    Insect

    Promoter

    Ubi-p63e

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUb FLAG-Human IntS6L

    Plasmid

    #198409

    Purpose

    Expresses FLAG-tagged human IntS6L

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator complex subunit 6 like (INTS6L Human)
    

    Tags

    3x FLAG

    Expression

    Insect

    Promoter

    Ubi-p63e

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EFS-DIO-GRAB_NTS1.0

    Plasmid

    #208671

    Purpose

    Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. 8xLexA-binding elements/pMD20

    Plasmid

    #48807

    Purpose

    Contains 8xLexA-binding elements

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2011;6(10):e26109. doi: 10.137

    Insert

    8xLexA-binding elements
    

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1 IntS6 AA 1035-1284

    Plasmid

    #196904

    Purpose

    Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 6 (IntS6 Fly)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet-1 IntS11 AA 300-597

    Plasmid

    #199329

    Purpose

    Expresses His-tagged Drosophila IntS11 amino acids 300-597

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 11 (IntS11 Fly)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 300-597 only

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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