We narrowed to 29,564 results for: ple

1. pLenti_EF1a_EV

   Plasmid

   #183116

   Purpose

   Empty vector control for pLenti_EF1a constructs

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   Small multiple cloning site
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiGuide

   Plasmid

   #117986

   Purpose

   pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

   Depositor

   Paul Khavari

   Article

   Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

   Insert

   sgRNA backbone (F+E)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv2_mSTING_gRNA_1

   Plasmid

   #196625

   Purpose

   Knock-out STING in murine cells, gRNA 1.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING gRNA 1 (Sting1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCMV_HyPer7RgDAAO

   Plasmid

   #217653

   Purpose

   Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Use

   Lentiviral

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Promoter

   CMV

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv2_mSTING_scrambled_gRNA_1

   Plasmid

   #196627

   Purpose

   Scrambled control 1 for STING knock-out in murine cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING scrambled gRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX_305

   Plasmid

   #41390

   Purpose

   Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

   Depositor

   David Root

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR_zeo_sgNT135

   Plasmid

   #183181

   Purpose

   pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6.3/EV

   Plasmid

   #120848

   Purpose

   Control vector, 20nt random sequence between gateway recombination sites

   Depositor

   Jan Rehwinkel

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLENTI_hACE2_PURO

   Plasmid

   #155295

   Purpose

   Lentiviral vector to generate hACE2 stable expressing cell line

   Depositor

   Raffaele De Francesco

   Insert

   hACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   C9

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLenti NL

    Plasmid

    #113450

    Purpose

    Lentiviral NanoLuc control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    cmyc

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_P2A_Puro

    Plasmid

    #211364

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Article

    Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR_mCherry

    Plasmid

    #229078

    Purpose

    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1s

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiRNAGuide_003

    Plasmid

    #192505

    Purpose

    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX_306

    Plasmid

    #41391

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_C_Myc_DDK_IRES_Puro_TARBP2

    Plasmid

    #106119

    Purpose

    Lentiviral expression of TARBP2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TARBP2 (TARBP2 Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and Myc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mApple-N1

    Plasmid

    #54567

    Purpose

    Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPR_Puro_sgTP53

    Plasmid

    #187819

    Purpose

    pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgTP53
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCMVblast_SLC38A2_wt

    Plasmid

    #156180

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA se…

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_CamKIIa_GFP

    Plasmid

    #96941

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    Green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPRv2_mSTING_gRNA_2

    Plasmid

    #196626

    Purpose

    Knock-out STING in murine cells, gRNA 2.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING gRNA 2 (Sting1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge