We narrowed to 18,696 results for: STO;

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pCAG-b3STOPb3-ZsGreen

Plasmid

#51266

Purpose

B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

b3STOPb3-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Nostoc-bGSDM-WELQ

Plasmid

#222100

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Nostoc-bGSDM-WELQ ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 No Stop

Plasmid

#84888

Purpose

DONOR vector for Gateway cloning of EWSR1 No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

no stop

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
B270 + SMARCAL1 sgSTOP

Plasmid

#100717

Purpose

B270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting SMARCAL1 (cloned using BbsI) and ATP1A1 (SMARCAL1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-SAR1-no stop

Plasmid

#67345

Purpose

hSar1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA-no stop

Plasmid

#67347

Purpose

hSara without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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B52 + SPRTN sgSTOP

Plasmid

#100709

Purpose

B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting SPRTN (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-STOP

Plasmid

#140876

Purpose

Multisite Gateway middle entry vector for expression of untagged mScarlet.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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B270 + SPRTN sgSTOP

Plasmid

#100718

Purpose

B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting SPRTN (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR2-HA Cterm

Plasmid

#84489

Purpose

overexpression of CASTOR2 with HA tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 (GATSL2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223 LC3B no-stop

Plasmid

#123205

Purpose

Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A no-stop

Plasmid

#123204

Purpose

Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-TAF15 No Stop

Plasmid

#84896

Purpose

DONOR vector for Gateway cloning of TAF15 No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway donor

Mutation

No Stop

Promoter

none

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-ccdB-6stop

Plasmid

#203511

Purpose

Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-ccdB-6stop

Plasmid

#203514

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-ccdB-6stop

Plasmid

#203512

Purpose

Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-1x-ccdB-6stop

Plasmid

#203519

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-2x-uA-ccdB-6stop

Plasmid

#203517

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-LynEGFP

Plasmid

#133923

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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