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pPOTv7-g418-mScarlet

Plasmid

#179823

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-nanoluc

Plasmid

#179797

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

nanoluc

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pICH71421

Plasmid

#51834

Purpose

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

plant 3'UTRpolyadenylation signal/terminator, Tom H4

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-eYFP

Plasmid

#190220

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

eYFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pICH42211

Plasmid

#50255

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter nos (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50011

Plasmid

#50317

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, EOS photoactivatable fluorescent protein (L. hemprichii)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80001

Plasmid

#50326

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, luciferase (P. pyralis)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mCherry

Plasmid

#190262

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCherry

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFP no-pA

Plasmid

#80819

Purpose

CMVmin-promoted GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-GFP no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL80004

Plasmid

#50325

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-BFP

Plasmid

#190258

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6.1-phleo-3Myc:mNG:3Myc

Plasmid

#181937

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-10Ty

Plasmid

#179807

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xTy

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-GFPmem no-pA

Plasmid

#80817

Purpose

GFPmem without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFPcaax no pA p3E

Use

Tags

Expression

Mammalian

Mutation

Promoter

GFP

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSPTg.T2FXK (#54)

Plasmid

#35963

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter
Tie2 enhancer

Use

Tags

Expression

Mutation

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pICSL80016

Plasmid

#50332

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, beta-glucuronidase (E. coli) with two introns

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTalpha1-EGFP-N1

Plasmid

#133929

Purpose

Neuron-specific expression of EGFP

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

EGFP

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-10Ty

Plasmid

#179808

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xTy

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-1xSYN-tTA

Plasmid

#19980

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

SYN-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pPRxRF3

Plasmid

#138386

Purpose

TagRFP-T knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

TagRFP-T

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge