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pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Promoter

2xCLEAR

Available since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDW363

Plasmid

#8842

Depositor

David Waugh

Publication

Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

Type

Empty backbone

Tags

MBP and biotin acceptor peptide

Expression

Bacterial

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

Plasmid

#149722

Purpose

Lentiviral expression of crRNA with GFP and a neomycin resistance gene

Depositor

Junwei Shi

Publication

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCR-Flag-IKKbeta-KM

Plasmid

#15466

Depositor

Hiroyasu Nakano

Publication

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IkB kinase beta-KM (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed Lysine at 44 to Alanine

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
mNeonGreen-Rab7

Plasmid

#129603

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab7

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHL-FcHis

Plasmid

#99846

Purpose

mammalian expression with secretive signal and FC tag

Depositor

Edith Yvonne Jones

Publication

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Tags

Fc_His

Expression

Mammalian

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28-hTDG

Plasmid

#70758

Purpose

bacterial expression of human TDG

Depositor

Primo Schaer

Publication

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

TDG (TDG Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSMP-EZH2_1

Plasmid

#36387

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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FCHo1-pmCherryC1

Plasmid

#27690

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FCHo1 (Fcho1 Mouse)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pBD-0001

Plasmid

#12373

Depositor

Axel Brunger

Publication

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP (Vcp Mouse)

Tags

His6

Expression

Bacterial

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHAGE-TO-dCas9

Plasmid

#75381

Purpose

dCas9

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Sp dCas9

Use

Lentiviral

Tags

no

Expression

Mammalian

Promoter

CMV-TO

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCXLE-Fbx15-cont2

Plasmid

#27081

Depositor

Shinya Yamanaka

Publication

Okita et al Nat Methods. 2011 May;8(5):409-12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FBXO15 (FBXO15 Human)

Expression

Mammalian

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His6 ProteinG TEV LIC cloning vector (1P)

Plasmid

#29660

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, Protein G, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Publication

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pLTR-G

Plasmid

#17532

Depositor

Jakob Reiser

Publication

Reiser et al Proc Natl Acad Sci U S A. 1996 Dec 24. 9

Insert

VSV-G glycoprotein (Indiana strain)

Use

Lentiviral

Expression

Mammalian

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pMAL-7xHis-TEV-hexa

Plasmid

#222868

Purpose

Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

Depositor

Nikolaus Loening

Publication

Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

Insert

TEV protease

Tags

7xHis and MBP

Expression

Bacterial

Mutation

C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-PIPK1 gamma 87

Plasmid

#22300

Depositor

Pietro De Camilli

Publication

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCR-HA-IKKbeta

Plasmid

#15470

Depositor

Hiroyasu Nakano

Publication

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IkB kinase beta (Ikbkb Mouse)

Tags

HA

Expression

Mammalian

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Spectrin-M-cpstFRET

Plasmid

#61109

Purpose

Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

Depositor

Fred Sachs

Publication

Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

spectrin-cpstFRET

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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