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p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Tags

flag

Expression

Mammalian

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pB-rtTA

Plasmid

#126034

Purpose

PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

Depositor

Mauro Calabrese

Article

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Insert

rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735

Use

Piggybac

Expression

Mammalian

Promoter

hUbiC

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSGKP-spe

Plasmid

#117234

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA-UL

Plasmid

#108551

Purpose

Bacterial Target-AID UGI-LVA vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1-UGI-LVA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
p3s-Sniper-Cas9

Plasmid

#113912

Purpose

Expresses Sniper-Cas9 with CMV promoter

Depositor

Jungjoon Lee

Article

Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

Insert

Sniper-Cas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCas9-CtIP

Plasmid

#109403

Purpose

Expresses Cas9 fusion to human CtIP protein

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

CtIP (RBBP8 Human)

Mutation

full length CtIP

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-mCherry

Plasmid

#108122

Purpose

Recruitable fluorophore

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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EB3-mScarlet-I

Plasmid

#98826

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs ABCDE>A

Plasmid

#83318

Purpose

human DNA-PKcs/ABCDE>ala

Depositor

Katheryn Meek

Article

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

6 phosphorylation sites in the ABCDE cluster subs…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA_NptII_Della

Plasmid

#91694

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-I-PhoCl-HCVp

Plasmid

#87697

Purpose

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pME-loxP-mTagBFP2-stop-pA-loxP

Plasmid

#75158

Purpose

Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

loxP-mTagBFP2-stop-pA-loxP

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCTCON2 Aga2P-HRP

Plasmid

#73152

Purpose

Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Aga2P-HRP

Tags

HA tag and myc tag

Expression

Yeast

Promoter

GAL1

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pZ8-T_dCas9

Plasmid

#74062

Purpose

pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-Dnmt3a3L-sc27

Plasmid

#71827

Purpose

For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

Depositor

Albert Jeltsch

Article

Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

Insert

Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)

Tags

6xHis tag

Expression

Bacterial

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Tags

GFP and Halotag

Expression

Mammalian

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU6a

Plasmid

#66194

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6a

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FuG-B2

Plasmid

#67513

Purpose

This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

Depositor

Kazuto Kobayashi

Article

Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

Insert

Fusion Glycoprotein type B2

Use

Lentiviral

Tags

Fusion protein of Vesicular stomatitis Indiana vi…

Promoter

CAGGS

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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