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pET30a BCP1 NTase

Plasmid

#195245

Purpose

Expresses phage BCP1 NTase

Depositor

Douglas Feldman

Article

Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

Insert

BCP1 phage NTase

Tags

His6 and SBP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a-Av05 NTase

Plasmid

#195243

Purpose

Expresses phage Av05 NTase

Depositor

Douglas Feldman

Article

Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

Insert

phage Av05 NTase

Tags

His6 and SBP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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ER-G-Ca-FLITS-KDEL

Plasmid

#191464

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

ER and KDEL

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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3xnls-G-Ca-FLITS

Plasmid

#191463

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

3xNLS

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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NaVSp1 DeltaCC pIRES

Plasmid

#128084

Purpose

Mammalian cell expression vector for NaVSp1 1-236 chimera bacterial sodium channel.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Expression

Mammalian

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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HA tag NaVSp1 FL pIRES

Plasmid

#128087

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel. N-terminal HA tag.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Tags

HA

Expression

Mammalian

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dN

Plasmid

#176465

Purpose

express VPS13D lacking N-terminal 117 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRDAX-391 HA UnaG[1] IRES UnaG[2]

Plasmid

#130139

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

UnaG

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-393 HA Venus210[1] IRES Venus210[2]

Plasmid

#130141

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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fADL-1e-HA-linker-p3

Plasmid

#139440

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, HA-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-CAG-R3

Plasmid

#141036

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

CAG promoter

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-tTA-ER-C3

Plasmid

#134568

Purpose

Expresses a YFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

tTA-ER

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-PtH

Plasmid

#133391

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-BtH

Plasmid

#133392

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMK-FLARE16-S aadA-GFP

Plasmid

#123562

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

FLARE16-S aadA-GFP

Use

Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDRFLIP42

Plasmid

#65518

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-Cit-attR1 and attR2-mCer-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP35

Plasmid

#65513

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-TFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAWV

Plasmid

#62710

Purpose

Donor vector for amiR-eGFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP123/amiR-eGFP419/FLPe

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pATI (pKD-G7)

Plasmid

#62686

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP32

Plasmid

#65511

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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