We narrowed to 32,755 results for: eng

1. pcDNA3.3-hALDOA K230A

   Plasmid

   #210811

   Purpose

   To block the formation of the initial Schiff base with FBP

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 230 of ALDOA mutated to alanine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBoBi-hALDOA D34S

   Plasmid

   #210807

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGIG-FLAG-SHTN1LRRR>GGG

   Plasmid

   #209570

   Purpose

   Mammalian expression of Shtn1 long isoform with RG mutation

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)
   

   Expression

   Mammalian

   Mutation

   RRR to GGG

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Coexpressed VLP NES

   Plasmid

   #182433

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Coexpressed VLP NES (M)

   Plasmid

   #182435

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(M)
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Coexpressed VLP NES (G)

   Plasmid

   #182477

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(G)
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Donor_C_FH_VIR

   Plasmid

   #186660

   Purpose

   Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Donor_N_FH_Shu

   Plasmid

   #186655

   Purpose

   Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Donor_C_FH_Nbr

   Plasmid

   #186662

   Purpose

   Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDonor_N_FH_GLE

    Plasmid

    #186657

    Purpose

    Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
    

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV V5-Chpt1

    Plasmid

    #175155

    Purpose

    Lentiviral expression of V5-tagged mouse Chpt1

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Chpt1 (Chpt1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV Dnajc16-V5

    Plasmid

    #175156

    Purpose

    Lentiviral expression of mouse Dnajc16-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Dnajc16 (Dnajc16 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV Cdipt-V5

    Plasmid

    #175144

    Purpose

    Lentiviral expression of mouse Cdipt-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Cdipt (Cdipt Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3xFLAG-CMV-Drosha(W1258)

    Plasmid

    #171535

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Expression

    Mammalian

    Mutation

    a Drosha stopping at W1258

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV Myc-emerin

    Plasmid

    #175101

    Purpose

    Lentiviral expression of Myc-tagged mouse Emd

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Emd (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3xFLAG-CMV-Drosha (P426)

    Plasmid

    #171537

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Expression

    Mammalian

    Mutation

    a Drosha mutant starting from P426

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3xFLAG-CMV-Drosha (E419)

    Plasmid

    #171534

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Expression

    Mammalian

    Mutation

    a Drosha mutant stopping at E419

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCK-Drosha-FLAG(delta6)

    Plasmid

    #171532

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Expression

    Mammalian

    Mutation

    small deletions of Drosha_1271A to 1276G_

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T-1-Dicer-dsRBD (+6)

    Plasmid

    #171544

    Purpose

    Encodes a Dicer fragment extending from from R1841 to the C-terminus

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    DICER1 (DICER1 Human)
    

    Expression

    Bacterial

    Mutation

    a six amino acid_TRLTEG_insertion in the L1 region

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Tac-STC

    Plasmid

    #162490

    Purpose

    Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its stem region, TM…

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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