We narrowed to 13,392 results for: mpl

1. DJ-1 TEV site pET15b

   Plasmid

   #60687

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   TEV site was inserted (see comments below).

   Promoter

   T7

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Nanog-luciferase targeting construct

   Plasmid

   #26591

   Depositor

   Rudolf Jaenisch

   Article

   Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

   Insert

   Nanog promoter-luciferase-lox-pgk-neomycin-loc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHisAlix deltaPRD

   Plasmid

   #42577

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Bacterial

   Mutation

   silent mutation A1617G (numbering in ALIX gene)

   Promoter

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pXPA-fapO-RgTALsyn

   Plasmid

   #100946

   Purpose

   Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   GAP (Constitutive)

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SPORT6-RNF114-UIMmut

   Plasmid

   #69923

   Purpose

   Expresses mutant myc-RNF114 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

   Insert

   RNF114 (RNF114 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S224P

   Promoter

   CMV

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAcrF2

   Plasmid

   #89234

   Purpose

   Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

   Depositor

   Blake Wiedenheft

   Article

   Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

   Insert

   AcrF2
   

   Use

   Tags

   6xHis-TEV

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5 HA-Arp8

   Plasmid

   #19796

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

   Insert

   Arp8 (ACTR8 Human)
   

   Use

   Tags

   2XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

   Plasmid

   #84260

   Purpose

   Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgSV40
   PYL1-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and mouse U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-DEST51 GFP-PNRC1

   Plasmid

   #123295

   Purpose

   Mammalian expression of PNRC1 N-GFP

   Depositor

   Giovanni Tonon

   Article

   Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

   Insert

   PNRC1 (PNRC1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159870

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPPAT-2XStrep

    Plasmid

    #125620

    Purpose

    expresses human PPAT protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneo-V5-SBP-MBP-HsNot4_64-575_AG

    Plasmid

    #148766

    Purpose

    Mammalian Expression of HsNot4_64-575

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4_64-575 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-MED6

    Plasmid

    #15411

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED6 (Med6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. GFP-BICD2N

    Plasmid

    #64070

    Purpose

    N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)

    Depositor

    Andrew Carter

    Article

    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

    Insert

    BICD2N (1-400) (Bicd2 Mouse)
    

    Use

    Tags

    GFP and His-ZZ-TCS

    Expression

    Insect

    Mutation

    Codon optimized for Sf9 expression

    Promoter

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p6897 PHAGE-P CMVT N-HA-hE6AP III wt

    Plasmid

    #37605

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP III (UBE3A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV tet regulatable

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pKM294

    Plasmid

    #134179

    Purpose

    Mammalian expression of CENP-C and KNL2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    mcherry-CENP-C 510-end-KNL2 (CENPC Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBacPAK8-His-myc-Rbx1

    Plasmid

    #29506

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Rbx1 (Rbx1 Mouse)
    

    Use

    Tags

    His and myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pfastbacHTb/mClock26-384

    Plasmid

    #47336

    Purpose

    mClock fragment cloned into pfastbacHTb vector with Hexa His tag

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    His and Tev

    Expression

    Mutation

    aa 26-384

    Promoter

    PH

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCIneo-lambdaN-HA-HsSmg6_I

    Plasmid

    #146551

    Purpose

    Mammalian Expression of HsSmg6

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsSmg6 (SMG6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations T594C, A699G and two non sil…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. KIF5C(1-560)-mCitrine

    Plasmid

    #61676

    Purpose

    Single mCitrine-labeled, truncated kinesin-1

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KHC(1-560)-mCitrine (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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