We narrowed to 11,578 results for: ALP

1. KG#65

   Plasmid

   #110933

   Purpose

   Expresses proteins in cholinergic neurons in the ventral nerve cord of C. elegans

   Depositor

   Kenneth Miller

   Article

   Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

   Inserts

   unc-17 beta promoter
   unc-54 3' control region
   

   Expression

   Bacterial

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGFP Glra2 KA (CC#303)

   Plasmid

   #15201

   Depositor

   Connie Cepko

   Article

   Young et al Neuron. 2004 Mar 25. 41(6):867-79.

   Insert

   Glra2 (GLRA2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K310A

   Available Since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

3. FR_luc

   Plasmid

   #31098

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   firefly luciferase
   

   Use

   Flp/frt

   Expression

   Mammalian

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

4. a5-ecto-pcDNA31hygro

   Plasmid

   #113386

   Purpose

   Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

   Depositor

   Timothy Springer

   Article

   Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

   Insert

   human alpha 5 ectodomain
   

   Tags

   Strep II tag

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 S100A1

   Plasmid

   #67048

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   S100A1 (S100A1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pRS424-TUB1-Cterminal6xHis

   Plasmid

   #60387

   Purpose

   Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Budding Yeast)
   

   Tags

   6xHIS

   Expression

   Yeast

   Promoter

   GAL

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLPS-hAkt1-gfp

   Plasmid

   #49388

   Purpose

   mammalian expression of human Akt1 fused to EGFP

   Depositor

   Randall McKinnon

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Cre/Lox; Acceptor vector

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   tga to cca at nt 1725 with respect to GenBank ID …

   Promoter

   CMV

   Available Since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

8. a4 ecto pcDNA3.1 hygro(-)

   Plasmid

   #113406

   Purpose

   expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

   Depositor

   Timothy Springer

   Article

   Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

   Insert

   human alpha 4 ectodomain
   

   Tags

   Strep II tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG416GPD-SEC7-Cerulean

   Plasmid

   #18851

   Depositor

   Susan Lindquist

   Article

   Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   SEC7 (SEC7 Budding Yeast)
   

   Tags

   Cerulean

   Expression

   Yeast

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pBacPAK8-VHL

    Plasmid

    #29498

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Von Hippel-Lindau (VHL Human)
    

    Expression

    Insect

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pRevLCav2

    Plasmid

    #60806

    Purpose

    used to make mutations in yeast REV3

    Depositor

    Youri Pavlov, Thomas Kunkel

    Article

    Pavlov et al Genetics. 2001 Sep;159(1):47-64.

    Insert

    REV3 (REV3 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    contains 647 C-terminal amino acids and 335 bp of…

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG416GPD-Cerulean-YPT52

    Plasmid

    #18843

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT52 (YPT52 Budding Yeast)
    

    Tags

    Cerulean

    Expression

    Yeast

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pAG416GPD-Cerulean-YPT1

    Plasmid

    #18848

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT1 (YPT1 Budding Yeast)
    

    Tags

    Cerulean

    Expression

    Yeast

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pAG416GPD-Cerulean-YPT6

    Plasmid

    #18845

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT6 (YPT6 Budding Yeast)
    

    Tags

    Cerulean

    Expression

    Yeast

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pDNR-hAkt1

    Plasmid

    #49387

    Purpose

    donor vector for cloning human Akt1 with 6xHN tag

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Donor vector

    Tags

    6xHN (6 repeating His-Asn subunits)

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    none

    Available Since

    Dec. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pLL3.7 Alk.1 shRNA

    Plasmid

    #59296

    Purpose

    shRNA to mouse Alk

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

    Insert

    Alk.1 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

17. KG#80

    Plasmid

    #110875

    Purpose

    Expresses the C. elegans acy-1 cDNA in body wall muscle

    Depositor

    Kenneth Miller

    Article

    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

    Inserts

    myo-3 promoter
    acy-1 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL3.7 Alk.2 shRNA

    Plasmid

    #59297

    Purpose

    shRNA to mouse Alk

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

    Insert

    Alk.2 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pmTgfa UTR

    Plasmid

    #31256

    Depositor

    David Lee

    Article

    Kornblum et al J Comp Neurol. 1997 Apr 7. 380(2):243-61

    Insert

    Tgfa (Tgfa Mouse)
    

    Use

    Cdna for in situ hybridization

    Mutation

    Exon 6 of Tgfa with 3'UTR

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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