207,468 results

1. pVDL9.3

   Plasmid

   #168299

   Purpose

   Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

   Depositor

   Víctor de Lorenzo

   Article

   Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

   Insert

   Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
   

   Expression

   Bacterial

   Promoter

   LacI-Plac

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-pcDNA3.1 Puro-CAG-Voltron2

   Plasmid

   #172909

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2
   

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. V. natriegens NC7

   Bacterial Strain

   #215356

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

4. CAV1-mCherry

   Plasmid

   #27705

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Tags

   monomeric Cherry

   Expression

   Mammalian

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMS-II-PEST

   Plasmid

   #204766

   Purpose

   GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-PEST-m6Alinker-DHFR
   

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-EGFP-C1-HsEDC3

   Plasmid

   #25032

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   EDC3 (EDC3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. YAP/TAZ-TRE-mStrawberry reporter

   Plasmid

   #158682

   Purpose

   This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CaMPARI

   Plasmid

   #60421

   Purpose

   Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI
   

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

    Plasmid

    #85434

    Purpose

    Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Pembrolizumab
    

    Use

    Lentiviral

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPW3758 : CMVd3-HsIRE1-HaloTag

    Plasmid

    #185680

    Purpose

    Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-Soma-jGCaMP8s (AAV9)

    Viral Prep

    #169256-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8s (#169256). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8s plasmid DNA. hSyn-driven expression of soma-targeted calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

16. AAV-PR rep/cap

    Plasmid

    #197565

    Purpose

    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

    Depositor

    Casey Maguire

    Article

    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

    Inserts

    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    

    Use

    AAV

    Mutation

    contains 7mer insert PRPPSTH between amino acids …

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMV306hsp

    Plasmid

    #26155

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    hsp60 promoter
    

    Use

    Mycobacteria integrating vector

    Available Since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-SFFV-KRAB-dCas9-P2A-mCherry

    Plasmid

    #60954

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Insert

    KRAB-dCas9-P2A-mCherry fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, KRAB domain, and mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pλPR cI-Elysis

    Plasmid

    #176891

    Purpose

    Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

    Depositor

    James Inglese

    Article

    Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

    Insert

    Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. GALT-SEP-mRuby3

    Plasmid

    #200938

    Purpose

    trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    GalT (B4GALT1 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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