We narrowed to 22,703 results for: TES

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pSIN-Ski-gRNA1

Plasmid

#180368

Purpose

targeting mouse Ski gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Ski targeting gRNA (Ski Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSN526

Plasmid

#184827

Purpose

Expresses human KIF5A(∆exon27) in insect cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (∆exon27) (KIF5A Human)

Tags

mScarlet-2xStrepII

Expression

Insect

Mutation

∆exon27 form of human KIF5A

Promoter

polyhedrin

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-woolly-monkey-retroCHMP3(148)-HA

Plasmid

#154250

Purpose

expresses prematurely truncated woolly monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-pygmy-marmoset-retroCHMP3(148)-HA

Plasmid

#154251

Purpose

expresses prematurely truncated pygmy marmoset retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV_Hyg_FLAG-BirA-eGFP-NLS2

Plasmid

#170917

Purpose

Expresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull down

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

FLAG-BirA-eGFP-NLS2

Tags

BirA-FLAG

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago2

Plasmid

#170918

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago1

Plasmid

#170919

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D

Plasmid

#176462

Purpose

expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D N3521S

Plasmid

#176463

Purpose

expressing VPS13D patient mutant N3521S in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Mutation

N3521S mutation

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV491

Plasmid

#177699

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV488

Plasmid

#177696

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_102-680_D

Plasmid

#146124

Purpose

Insect Expression of DmEDC3_102-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_102-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_331-680_D

Plasmid

#146125

Purpose

Insect Expression of DmEDC3_331-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_331-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Pigb-V5

Plasmid

#175160

Purpose

Lentiviral expression of mouse Pigb-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Pigb (Pigb Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mSpata33-PA

Plasmid

#179129

Purpose

Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (Spata33 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTF

Plasmid

#162784

Purpose

Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

Depositor

Ivan Vorobiev

Article

Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

Type

Empty backbone

Tags

10xHis and FLAG

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+WBP2NL-HA

Plasmid

#174791

Purpose

Xenopus laevis WBP2NL with 3'HA tag

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

WBP2 N-terminal like (wbp2nl.L Frog)

Tags

HA

Expression

Bacterial

Mutation

aa#165, Y to H; aa# 287, D to H

Promoter

SP6

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2S31A

Plasmid

#174332

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-NKD2S31A

Plasmid

#174331

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

MycHis

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nl

Plasmid

#172463

Purpose

expresses wild-type protein when injected into embryos

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl (wbp2nl.L Frog)

Use

Xenopus expression

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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UUUUA-pHG165c

Plasmid

#170107

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

UUUUA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MMMMA-pHG165c

Plasmid

#170108

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

MelR

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA-pHG165c

Plasmid

#170110

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA_Q182A_R287H-pHG165c

Plasmid

#170111

Purpose

Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA_Q182A_R278H

Expression

Bacterial

Mutation

Q182A, R287H

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RRRAA-pHG165c

Plasmid

#170112

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

RRRAA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin-TAPBPR 22-35

Plasmid

#153478

Purpose

Mammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

Tapasin a.a.10-20 replaced with TAPBPR a.a. 22-35

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin delta2step

Plasmid

#153479

Purpose

Mammalian expression of FLAG-tagged Tapasin delta2step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.13-14, 16-17

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin delta3step

Plasmid

#153480

Purpose

Mammalian expression of FLAG-tagged Tapasin delta3step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.12-14, 16-18

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin G15E

Plasmid

#153481

Purpose

Mammalian expression of FLAG-tagged Tapasin G15E

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

G15E

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin G15L

Plasmid

#153482

Purpose

Mammalian expression of FLAG-tagged Tapasin G15L

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

G15L

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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