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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 6101 - 6120 of 17592 results

  4. ZJOM5

    Plasmid
    #133635
    Purpose
    Integration of CHS5-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-CHS5-GFP (CHS5 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133635-plasmid-map-sequence-id-267441

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  5. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid
    #133318
    Purpose
    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pasteurianus)
    Use
    Unspecified
    Promoter
    xylose
    Available Since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    133318-plasmid-map-sequence-id-266833

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  10. Kaede-Nucleus-9

    Plasmid
    #57319
    Purpose
    Localization: Nucleus, Excitation: 508 / 572, Emission: 518 / 580
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    Kaede-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. tdTomato-PLK1-N-16

    Plasmid
    #58125
    Purpose
    Localization: Centrosomes, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    PLK1 (PLK1 Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. ZJOM86

    Plasmid
    #133634
    Purpose
    Integration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-ANP1-GFP (ANP1 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133634-plasmid-map-sequence-id-265885

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  16. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  17. pUASTattb-cAMPr Y205A, R626A

    Plasmid
    #84932
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R626A
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R626A
    Promoter
    5X UAS
    Available Since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84932-plasmid-map-sequence-id-180302

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  20. mPlum-Rab7a-7

    Plasmid
    #55997
    Purpose
    Localization: Ras GTPase, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    Rab7a (RAB7A Human)
    Tags
    mPlum
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6101 - 6120 of 17592 results