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pAM002

Plasmid

#170006

Purpose

Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PpmrD

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NASP-Halo

Plasmid

#175340

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NASP (NASP Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFD-FKBP12

Plasmid

#137108

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

FKBP12

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBA2257

Plasmid

#38151

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

MBP-proMeCPA fusion protein

Tags

maltose binding protein

Expression

Bacterial

Promoter

tac

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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CasRx gRNA targeting EZH2

Plasmid

#219655

Purpose

hU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

CasRx gRNA targeting EZH2

Use

CRISPR

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-p110α-CAAX

Plasmid

#39490

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Tags

CAAX and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSYC-181

Plasmid

#31566

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

pCS4+-P2A

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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NM R(delta) 2-5 GFP

Plasmid

#1209

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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PCLP-2/NT-IgG (104)

Plasmid

#24423

Depositor

Steven Rosen

Article

Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

Insert

PCLP-2 (PODXL2 Human)

Tags

Human IgG1 Fc

Expression

Mammalian

Mutation

N-terminal (acidic) domain of PCLP-2 (1-630 bp) f…

Available Since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only
pNOC-RLUC

Plasmid

#98142

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven renilla luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt8b

Plasmid

#42285

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8b (Wnt8b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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JB26

Plasmid

#128920

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab33BQL-BirA*-HA-MAO (RAB33B Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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p3992 pET ubcH5

Plasmid

#8652

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH5 (UBE2D1 Human)

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-GFP-CENPBpA

Plasmid

#120162

Purpose

EGFP followed by the poly(A) site from human CENPB gene

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDM767

Plasmid

#207425

Purpose

Extrachromosomal expression HSPC300-GFP and PH-CRAC-mRFPmars

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
CRAC

Use

Unspecified

Tags

GFP and mRFPmars

Mutation

aa 1–126 (PH domain) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDM1424

Plasmid

#207424

Purpose

Extrachromosomal expression HSPC300-GFP and PkgE-PH-mCherry

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
PkgE

Use

Unspecified

Tags

GFP and mCherry

Mutation

aa 1–100 (PH domain) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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JB15

Plasmid

#128918

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab30QL-BirA*-HA-MAO (RAB30 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSG 12-6

Plasmid

#63870

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only