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PRPF4B gRNA (BRDN0001146215)

Plasmid

#76431

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRPF4B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRPF4B

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK223 gRNA (BRDN0001146707)

Plasmid

#75791

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK223

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK223

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK223 gRNA (BRDN0001147337)

Plasmid

#75792

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK223

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK223

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK223 gRNA (BRDN0001147947)

Plasmid

#75793

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK223

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK223

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADRBK1 gRNA (BRDN0001144871)

Plasmid

#75681

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADRBK1 gRNA (BRDN0001145957)

Plasmid

#75682

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADRBK1 gRNA (BRDN0001147663)

Plasmid

#75683

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADRBK1 gRNA (BRDN0001147919)

Plasmid

#75684

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ZAK gRNA (BRDN0001146874)

Plasmid

#75598

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZAK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ZAK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRPF4B gRNA (BRDN0001146471)

Plasmid

#76430

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRPF4B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRPF4B

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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PMKO-sh_lnc-BATE1

Plasmid

#64866

Purpose

Expresses shRNA targeting lnc-BATE1

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

shRNA targeting lnc-BATE1

Use

Retroviral

Promoter

u6

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
Magoh198delG

Plasmid

#30481

Depositor

William Pavan, Debra Silver

Article

Silver et al Nat Neurosci. 2010 May . 13(5):551-8.

Insert

Magoh (Magoh Mouse)

Expression

Mammalian

Mutation

198delG (point nucleotide deletion)

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfSec15A_2

Plasmid

#26715

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Sec15A RNAi (EXOC6 C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Sec15A RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfSec15A_5

Plasmid

#26716

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Sec15A RNAi (EXOC6 C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Sec15A-specific RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pGTM_COV2_NSP3_003_SUMO

Plasmid

#233739

Purpose

Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease cleavage no nonnative residues remain on the PLpro protein

Depositor

Gaetano Montelione

Article

De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do

Insert

PLpro (ORF1ab SARS-CoV-2)

Tags

6xHis-Sumo

Expression

Bacterial

Promoter

T7 lac

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pDsRed1-CMV-Rat Arc-DsRed1-pA

Plasmid

#233049

Purpose

To Express a Rat Arc-DsRed fusion protein from a CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

Insert

rat Arc-DsRed fusion (Arc Rat)

Tags

DsRed1

Expression

Mammalian

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

Plasmid

#198337

Purpose

PB plasmid expressing Halo-tagged PP7 coat protein

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

PP7 coat protein 3x Halo-GB1

Use

Piggybac

Tags

HaloTag

Expression

Mammalian

Promoter

Ef1a

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET29_LuxSit_i

Plasmid

#194299

Purpose

Bacterial expression of the de novo luciferase, LuxSit-i

Depositor

David Baker, Andy Yeh

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Expression

Bacterial

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag003

Plasmid

#225194

Purpose

Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag003

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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