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pSG5-BMRF1

Plasmid

#72631

Purpose

Mammalian expression vector for Epstein-Barr virus polymerase accessory protein

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BMRF1

Tags

D22G and S349N

Expression

Mammalian

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
AaHsp70Aa-1373-FFluc

Plasmid

#52911

Purpose

Ae aegypti hsp70Aa promoter driving firefly luciferase

Depositor

Zach Adelman

Article

Gross et al J Med Entomol. 2009 May;46(3):496-504.

Insert

Aa Hsp70Aa-1373

Use

Luciferase

Promoter

Aa Hsp70Aa-1373

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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p3693 HPV-11 E1

Plasmid

#10860

Depositor

Peter Howley

Article

Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

Insert

HPV-11 E1

Expression

Mammalian

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.(cyto).iGlucoSnFR2

Plasmid

#244110

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS793

Plasmid

#238206

Purpose

Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

TwinStrepII-bdSUMO

Expression

Bacterial

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225239

Purpose

Mammalian expression of iPASnFR-H in mitochondria

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3-FR-2Strep

Plasmid

#210315

Purpose

Expresses SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3 (ORF1ab SARS-CoV-2)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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optoAKT

Plasmid

#207164

Purpose

Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway

Depositor

Kai Zhang

Article

Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554

Insert

CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX

Expression

Mammalian

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-tdTomato

Plasmid

#122101

Purpose

AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

tdTomato

Use

AAV

Tags

NA

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pOGG004

Plasmid

#113979

Purpose

Vector construction module. Level 1 golden gate cloning site module with LacZ reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Level 1 golden gate cloning site

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-EYFP-WPRE

Plasmid

#104104

Purpose

Expresses EYFP under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

EYFP

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FYN-1/1

Plasmid

#91413

Purpose

Protein expression and purification of human SH3 domain construct FYN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FYN-1/1 (FYN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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EBFP2-CathespinB-6

Plasmid

#55236

Purpose

Localization: Lysosomes, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Article

Sundaresan et al J Clin Oncol. 1987 Nov;5(11):1739-45.

Insert

CathespinB

Tags

EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-luxAB2

Plasmid

#27061

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfPar3_3

Plasmid

#26705

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Par3 RNAi

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
pVP64

Plasmid

#22287

Depositor

Louise Prakash

Article

Pages et al Genes Dev. 2009 Jun 15. 23(12):1438-49.

Insert

REV1 (REV1 Budding Yeast)

Tags

4xHA

Expression

Yeast

Available Since

April 22, 2010

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP4(essQ-cspB)

Bacterial Strain

#110244

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP6(ygcE-ygcF)

Bacterial Strain

#110246

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE (AAV1)

Viral Prep

#172908-AAV1

Purpose

Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE (#172908). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE plasmid DNA. Syn-driven, Cre-dependent Ace2N-4AA-mNeon-ST A122D expression. These AAV preparations are suitable purity for injection into animals.

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Promoter

Syn

Tags

mNeon (Cre-dependent)

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only