We narrowed to 8,923 results for: Mag

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GAL-p300-2-300

Plasmid

#89098

Purpose

mammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 2-300

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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Halo-Tev-Flag-Ube2v2

Plasmid

#110070

Purpose

Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

Depositor

Yimon Aye

Article

Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

Insert

Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)

Tags

Halo-TEV-Flag

Expression

Mammalian

Promoter

T7

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1901-2414

Plasmid

#89103

Purpose

mammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1901-2414

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-CAGGS (JDW 912)

Plasmid

#224472

Purpose

Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

CAGGS Promoter

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100850

Purpose

AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GACh2.0

Plasmid

#121921

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor GACh2.0

Use

AAV

Promoter

hSyn

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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POLR2A-N CRISPR pX330

Plasmid

#124495

Purpose

A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A targeting CRISPR

Use

CRISPR

Promoter

U6 promoter

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso2

Plasmid

#159586

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 2 in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-3xnls-Tq-Ca-FLITS

Plasmid

#145030

Purpose

piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Tags

3xnls-Tq-Ca-FLITS

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloEKAR2.2-T2A-EGFP

Plasmid

#216495

Purpose

Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloEKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

Plasmid

#134869

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome b5 type A (CYB5A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-98

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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Halo-Tev-Flag-Ube2v1

Plasmid

#110069

Purpose

Express Halo-Tev-Flag-Ube2v1 (human) in mammalian cells

Depositor

Yimon Aye

Article

Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

Insert

Halo-Tev-Flag-Ube2v1 (UBE2V1 Human)

Tags

Halo-TEV-Flag

Expression

Mammalian

Promoter

T7

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3 R186S

Plasmid

#175779

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Mutation

R186S

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Mito-mTurq2-Apollo-NADP+

Plasmid

#193303

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

mTurqoise2 and mitochondrial targeting sequence

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-HA-deLACCO1-NGR

Plasmid

#208097

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

AAV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGEN-WPRE-bGH-DEST (JDW 1309)

Plasmid

#229815

Purpose

A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM66

Plasmid

#65400

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM66 (TRIM66 Human)

Tags

GFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only