We narrowed to 15,317 results for: Vars;

1. pDJSVT86

   Plasmid

   #108965

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

   Type

   Empty backbone

   Use

   Tags

   OmpA 1-27, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(+)- PC-MjMAT

   Plasmid

   #179372

   Purpose

   PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

   Depositor

   Andrea Rentmeister

   Article

   Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

   Insert

   PC-MjMAT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L147A, I351A

   Promoter

   T7

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

   Plasmid

   #159853

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mNeon(PATC)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CMV-evoCjCas9-BE4max-BlastR

   Plasmid

   #194061

   Purpose

   Mammalian expression of evoCjCas9 CBE4max

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-flex-oChIEF-citrine

   Plasmid

   #50973

   Purpose

   flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   inverted and inserted between two lox sites

   Promoter

   human synapsin promoter

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p416 72Q GPD

   Plasmid

   #1179

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 72Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CamKIIa-moxBFP-WPRE

   Plasmid

   #194976

   Purpose

   AAV vector to drive expression of moxBFP under the control of CamKIIalpha promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   moxBFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-DUET-1-mRhubarb713-HO

   Plasmid

   #141199

   Purpose

   Expresses mRhubarb713 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb713
   heme oxygenase
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, W309R, Q310A compared to iRFP

   Promoter

   T7

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-R4-uAb

   Plasmid

   #101800

   Purpose

   Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

   Depositor

   Matthew DeLisa

   Article

   Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

   Insert

   R4-uAb
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-P3-evoCjCas9-TadCDd_gRNA

    Plasmid

    #202563

    Purpose

    P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-TadCDd
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. xABEmax

    Plasmid

    #119813

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1P shSHMT2

    Plasmid

    #106319

    Purpose

    Express shRNA targeting SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    shRNA targeting SHMT2
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHBJT001

    Plasmid

    #225152

    Purpose

    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-ar1

    Plasmid

    #225219

    Purpose

    Expression androgen receptor 1 in mammalian cells

    Depositor

    Deshou Wang

    Article

    Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

    Insert

    androgen receptor 1
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ppst93-ANME2bHR1 MCR-NG

    Plasmid

    #192774

    Purpose

    Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Ppst93-ANME1G37 MCR-NG

    Plasmid

    #192773

    Purpose

    Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. epB_UbC_tdMS2cp-tagRFP-Tx2

    Plasmid

    #119908

    Purpose

    ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    HA-tdMS2cp-tagRFP-Tx2-nls
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p416 103Q GAL

    Plasmid

    #1186

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CP1028-CBEmax

    Plasmid

    #119802

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1028) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXJ40-Flag-Ferritin-iBox-PAK4cat

    Plasmid

    #190180

    Purpose

    expresses Flag-tagged Ferritin-iBox-PAK4cat

    Depositor

    Bianxiao Cui

    Article

    Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

    Insert

    Ferritin-iBox-PAK4cat
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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