We narrowed to 81,726 results for: MYC

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pCMV-PE7

Plasmid

#214812

Purpose

Mammalian expression of SpCas9 PE7 prime editor

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6b

Plasmid

#207852

Purpose

Mammalian expression of PE6b prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6b

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax

Plasmid

#174820

Purpose

Mammalian expression of SpCas9 PEmax prime editor

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PB-PEmax

Plasmid

#187893

Purpose

All-in-one prime editor piggyBac transposon with PEmax

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-GFP/Cre

Plasmid

#49056

Purpose

Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, Myc, and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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Lentiviral-S-tdTomato

Plasmid

#112579

Purpose

Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

Depositor

Jeffrey Rothstein

Article

Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

Insert

NLS-tdTomato-NES

Use

Lentiviral

Tags

nuclear export sequence and nuclear localization …

Expression

Mammalian

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-PEmax for IVT

Plasmid

#178113

Purpose

Template for in vitro transcription of PEmax

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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T7_CC_PE7_IVT_Template

Plasmid

#223022

Purpose

Template for in vitro transcription of PE7. For HSPC-related experiments.

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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PEmax_AAVS1_knockin_HDR_donor

Plasmid

#223000

Purpose

HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PEmax

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLHCX-XBP1 mNeonGreen NLS

Plasmid

#115971

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Human, Synthetic)

Use

Retroviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6a

Plasmid

#207851

Purpose

Mammalian expression of PE6a prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6a

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-beta-catenin deltaN90

Plasmid

#36985

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

beta-catenin deltaN90 (CTNNB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90 (Constitutively Acti…

Promoter

EF1a

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6g

Plasmid

#207857

Purpose

Mammalian expression of PE6g prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6g

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6f

Plasmid

#207856

Purpose

Mammalian expression of PE6f prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6f

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-PDGFRA-reporter

Plasmid

#136456

Purpose

Mammalian fluorescent reporter plasmid for PDGFRA signaling.

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Inserts

PDGFRalpha (PDGFRA Human)
Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV and Minimal promoter with artificial SRE enha…

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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GABA(A) receptor subunit a1SE

Plasmid

#49168

Purpose

pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

Insert

GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)

Tags

myc and pHGFP (pHIuorin)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171617

Purpose

AAV Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

AAV and Synthetic Biology

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#101061

Purpose

AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2-F391W

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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