161,473 results

1. pFX-β-tubulin-mCherry

   Plasmid

   #175829

   Purpose

   Express mCherry with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with microtuble-targeting sequence
   

   Use

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. G13-CASE

   Plasmid

   #168127

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at F125/D126 within GNA13

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-FGFR1

   Plasmid

   #116740

   Purpose

   Lentiviral expression of FGFR1

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FGFR1 (FGFR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gs(short)-CASE

   Plasmid

   #168124

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG1

   Expression

   Mammalian

   Mutation

   NLuc is inserted at N136/V137 within GNAS

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9-GFP_sg_mTfeb

   Plasmid

   #79006

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   sgTfeb (Tfeb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gi1-CASE

   Plasmid

   #168120

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A121/E122 within GNAI1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-NES-KIRIN1

   Plasmid

   #107416

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gi3-CASE

   Plasmid

   #168122

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A114/E115 within GNAI1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR v2 sgKEAP1-3

   Plasmid

   #186837

   Purpose

   knock out KEAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

   Insert

   Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pInducer20 Cyclin E1

    Plasmid

    #109348

    Purpose

    virus packaged, doxycyline inducible expression of Cyclin E1

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    Cyclin E1 (CCNE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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