We narrowed to 32,471 results for: Eng

1. ARK1a

   Plasmid

   #81016

   Purpose

   E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)

   Depositor

   Taek Soon Lee

   Article

   Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

   Inserts

   MevTo-BBa1002-pTrc-Mkco-PMKco
   PMD-NudB
   

   Promoter

   LacUV5 and Trc promoter

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMY07AB-ORF

   Plasmid

   #127711

   Purpose

   Level 0 destination vector for a coding sequence with B - C scars. Clone in with BpiI

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMY07CD-TER

   Plasmid

   #127712

   Purpose

   Level 0 destination vector for a terminator with C - D scars. Clone in with BpiI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDSM-5a

   Plasmid

   #127716

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 1. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDSM-ab

   Plasmid

   #127717

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 2. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDSM-bc

   Plasmid

   #127718

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 3. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDSM-cd

   Plasmid

   #127719

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 4. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDSM-de

   Plasmid

   #127720

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDSM-ef

   Plasmid

   #127721

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDSM-f3

    Plasmid

    #127722

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

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11. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCKmatBC

    Plasmid

    #138587

    Purpose

    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    matB contains native GUG start codon

    Promoter

    Plac promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

    Plasmid

    #169524

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT1

    Plasmid

    #98299

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
    

    Expression

    Yeast

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAL-c5x_UnaG

    Plasmid

    #163125

    Purpose

    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    UnaG
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCN-mBFP

    Plasmid

    #63847

    Purpose

    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

    Depositor

    Howard Salis

    Article

    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

    Insert

    mBFP
    

    Expression

    Bacterial

    Mutation

    Promoter and RBS was swapped. His-tag was removed…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSMART-HCKan-yibDp-VHH-VCAM1

    Plasmid

    #202471

    Purpose

    Expresses the nanobody VCAM1 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VCAM1
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHYX137

    Plasmid

    #202814

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ScPCK1

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHYX138

    Plasmid

    #202815

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ScADH2

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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