We narrowed to 31,999 results for: Eng

1. pDSM-cd

   Plasmid

   #127719

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 4. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSM-de

   Plasmid

   #127720

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDSM-ef

   Plasmid

   #127721

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDSM-f3

   Plasmid

   #127722

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMAL-c5x_UnaG

   Plasmid

   #163125

   Purpose

   A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   UnaG
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHYX137

   Plasmid

   #202814

   Purpose

   Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

   Depositor

   Jean-Félix Dallery

   Article

   Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScPCK1

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHYX138

   Plasmid

   #202815

   Purpose

   Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

   Depositor

   Jean-Félix Dallery

   Article

   Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScADH2

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2374-Tier1-PhCMV-Gal4_VP16

   Plasmid

   #169582

   Purpose

   Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   GAL4-transactivator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCKmatBC

   Plasmid

   #138587

   Purpose

   matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   matB contains native GUG start codon

   Promoter

   Plac promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCN-mBFP

    Plasmid

    #63847

    Purpose

    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

    Depositor

    Howard Salis

    Article

    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

    Insert

    mBFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter and RBS was swapped. His-tag was removed…

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSMART-HCKan-yibDp-VHH-VCAM1

    Plasmid

    #202471

    Purpose

    Expresses the nanobody VCAM1 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VCAM1
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPPC019.AAV

    Plasmid

    #171147

    Purpose

    Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPPC005

    Plasmid

    #171140

    Purpose

    For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    XylS-Pm
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH64-Tier1-PSV40-Citrine_p2a_SEAP

    Plasmid

    #169558

    Purpose

    Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PSV40-driven citrine-P2A-SEAP fusion construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PSV40

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPMVAd3

    Plasmid

    #98298

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCas34

    Plasmid

    #82386

    Purpose

    sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT1

    Plasmid

    #98299

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NFkBr-IL1Ra

    Plasmid

    #183065

    Purpose

    Expresses IL-1Ra downstream of a synthetic promoter activated by the NFkB signaling pathway

    Depositor

    Farshid Guilak

    Article

    Pferdehirt et al Tissue Eng Part A. 2019 May;25(9-10):809

    Insert

    interleukin-1 receptor antagonist (Il1rn Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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