We narrowed to 651 results for: RP2

1. ACTR2 gRNA (BRDN0001146434)

   Plasmid

   #76955

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ACTR2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ACTR2 (ACTR2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AID-C12*-n'Cas9-BE4max

   Plasmid

   #216730

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-n'Cas9-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AID-C12*-dCas9-BE4max

   Plasmid

   #216731

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-dCas9-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBactin-Akna-mOrange2

   Plasmid

   #196865

   Purpose

   Expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-PSG1-COMP5AP-AviTag-9xHis

   Plasmid

   #157379

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PSG1 (PSG1 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CERKL gRNA (BRDN0001148191)

   Plasmid

   #77675

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CERKL

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CERKL (CERKL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CERKL gRNA (BRDN0001145669)

   Plasmid

   #77676

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CERKL

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CERKL (CERKL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CERKL gRNA (BRDN0001149167)

   Plasmid

   #77677

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CERKL

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CERKL (CERKL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ACTR2 gRNA (BRDN0001145581)

   Plasmid

   #76956

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ACTR2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ACTR2 (ACTR2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ACTR2 gRNA (BRDN0001145720)

    Plasmid

    #76957

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ACTR2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ACTR2 (ACTR2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINB-HOIL-1 full-length (1–510)

    Plasmid

    #193858

    Purpose

    Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

    Depositor

    Bernhard Lechtenberg

    Article

    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

    Insert

    HOIL-1 (RBCK1 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    Codon optimised for expression in E. coli

    Promoter

    T7

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

    Plasmid

    #153062

    Purpose

    Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutagenesis to insert a stop codon at aa 298 in B…

    Promoter

    EF1α

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL1 sgRNA(MS2)-MS2-AIDmono

    Plasmid

    #112127

    Purpose

    empty sgRNA cloning vector with MS2-AIDmono

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, H130A/R131E…

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORANGE GFP-Arpc5 KI

    Plasmid

    #131503

    Purpose

    Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Arpc5, Arpc5 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3 sgRNA(MS2)-MS2-AID3f

    Plasmid

    #112129

    Purpose

    empty sgRNA cloning vector with MS2-AID3f

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AIDmono 3f mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL2 sgRNA(MS2)-MS2-AID3c

    Plasmid

    #112128

    Purpose

    empty sgRNA cloning vector with MS2-AID3c

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono 3c mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_EYS_WT

    Plasmid

    #81854

    Purpose

    Gateway Donor vector containing EYS ,part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWZL Neo Myr Flag PLK4

    Plasmid

    #20522

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pDONR P2R-P3-IRES_ECFP

    Plasmid

    #48354

    Purpose

    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    IRES_ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    ECFP translation is mediated by an IRES. Contains…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_EYS_p.N269H

    Plasmid

    #81557

    Purpose

    Gateway Donor vector containing EYS ,part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    N269H

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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