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Plasmid#113655PurposesgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.DepositorInsertpromoter T5 and sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterT5Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG3H-U6SC
Plasmid#134755PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-35SCAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3K-U6SC
Plasmid#134754PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-35SCAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2NT
Plasmid#62257Purposeexpression of A2NT sgRNA from the arabinose-inducible promoterDepositorInsertA2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pG3B-U6SC
Plasmid#134753PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-35SCAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3B-U3Ub
Plasmid#134750PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA- OsU3, zCas9-Ubi1Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-scramble
Plasmid#62285Purposeexpression of scramble sgRNA from the arabinose-inducible promoterDepositorInsertscramble sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pG3H-U6EC1
Plasmid#134758PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-EC1Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3K-U6EC1
Plasmid#134757PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-EC1Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3B-U6EC1
Plasmid#134756PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-EC1Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3H-U3Ub
Plasmid#134749PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA- OsU3, zCas9-Ubi1Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
UseTagsExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2T
Plasmid#62258Purposeexpression of A2T sgRNA from the arabinose-inducible promoterDepositorInsertA2T sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A1T
Plasmid#62256Purposeexpression of A1T sgRNA from the arabinose-inducible promoterDepositorInsertA1T sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A4NT
Plasmid#62261Purposeexpression of A4NT sgRNA from the arabinose-inducible promoterDepositorInsertA4NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A3NT
Plasmid#62259Purposeexpression of A3NT sgRNA from the arabinose-inducible promoterDepositorInsertA3NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
ADEPT-pTarget-Msp4
Plasmid#238037PurposeEncodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterlacAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
ADEPT-pTarget-Msp2
Plasmid#238035PurposeEncodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterlacAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
ADEPT-pTarget-Msp3
Plasmid#238036PurposeEncodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterlacAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
ADEPT-pTarget-Msp6
Plasmid#238039PurposeEncodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterlacAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only