We narrowed to 953 results for: dcas9, grna

1. GG-dest

   Plasmid

   #69538

   Purpose

   Destination plasmid for gRNA concatenation Golden Gate cloning

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCD296.7

   Plasmid

   #113320

   Purpose

   dCas9, MCP-TetD, J107 scRNA.b1

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   dCas9, MCP-TetD, J107 scRNA.b1_MS2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCD294.9

   Plasmid

   #113319

   Purpose

   dCas9, PCP-SoxS_R93A, J109 scRNA.b1

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   dCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   R93A

   Promoter

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK422

   Plasmid

   #155373

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK423

   Plasmid

   #155374

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP712

   Plasmid

   #65768

   Purpose

   Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFD115

   Plasmid

   #124768

   Purpose

   pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   cas9
   term PpflB sgRNA (BsaI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ptet

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRnc-gGFP129

   Plasmid

   #189537

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-129-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOP587

    Plasmid

    #85944

    Purpose

    pOP576 with gRNA key

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9 and gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOP588

    Plasmid

    #85945

    Purpose

    pOP576 with gRNA key

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9 and gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOP589

    Plasmid

    #85946

    Purpose

    pOP576 with gRNA key

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9 and gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOP590

    Plasmid

    #85947

    Purpose

    pOP576 with gRNA key

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9 and gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOP591

    Plasmid

    #85948

    Purpose

    pOP576 with gRNA key

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9 and gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOP592

    Plasmid

    #85949

    Purpose

    pOP576 with gRNA key

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9 and gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOP593

    Plasmid

    #85950

    Purpose

    pOP576 with gRNA key

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9 and gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOP594

    Plasmid

    #85951

    Purpose

    pOP576 with gRNA key

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9 and gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK429

    Plasmid

    #155376

    Purpose

    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge