We narrowed to 759 results for: gc

1. HemH-R212A_pET-14b

   Plasmid

   #233262

   Purpose

   Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

   Depositor

   Mohan Babu, Andrew Emili

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   HemH (hemH )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 21…

   Promoter

   T7 promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCHA_OROV_NSm

   Plasmid

   #208940

   Purpose

   Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells

   Depositor

   Gerald Barry

   Insert

   NSm
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted 949 bp from 5' end (Gn), deleted 29…

   Promoter

   CMV

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

   Plasmid

   #172910

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2-ST
   

   Use

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGP-CAG-VARNAM A122D WPRE-bGH-polyA

   Plasmid

   #180486

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   VARNAM A122D
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GalToxBFP

   Plasmid

   #68073

   Purpose

   mammalian expression of GC localized oxBFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   GalT signal anchor transmembrane domain
   

   Use

   Tags

   moxBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pISH-Gucy1b2-probe1

   Plasmid

   #105454

   Purpose

   to generate a riboprobe for in situ hybridazation

   Depositor

   Peter Mombaerts

   Article

   Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

   Insert

   Gucy1b2 (Gucy1b2 Mouse)
   

   Use

   To ganerate riboprobe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pISH-Gucy1b2-probe2

   Plasmid

   #105455

   Purpose

   to generate a riboprobe for in situ hybridazation

   Depositor

   Peter Mombaerts

   Article

   Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

   Insert

   Gucy1b2 (Gucy1b2 Mouse)
   

   Use

   To ganerate riboprobe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DGP104- pSP64 Poly(A)700

   Plasmid

   #70098

   Purpose

   an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

   Depositor

   David Gresham

   Article

   Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

   Insert

   700bp insert from B. subtilis
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

9. DGP105- pSP64 Poly(A)900

   Plasmid

   #70100

   Purpose

   an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

   Depositor

   David Gresham

   Article

   Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

   Insert

   900bp insert from B. subtillis
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

10. DGP106- pSP64 Poly(A)1200

    Plasmid

    #70101

    Purpose

    an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

    Depositor

    David Gresham

    Article

    Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

    Insert

    1200bp insert from B. subtillis
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. SL-TREAT

    Plasmid

    #196918

    Purpose

    The TREAT reporter containing cap-proximal GC-rich stemloop

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

    Plasmid

    #172911

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Ace2N-4AA-mNeon A122D
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

    Plasmid

    #172912

    Purpose

    Mammalian expression of voltage sensor; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Ace2N-4AA-mNeon-ST A122D
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SL-SunTag

    Plasmid

    #196919

    Purpose

    The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MCV-hVam6p-

    Plasmid

    #32059

    Depositor

    Patrick Moore

    Article

    Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

    Insert

    MCV-hVam6p-
    

    Use

    Tags

    Expression

    Mutation

    TG to GC mutations at 3280-3281 (nt) of full cons…

    Promoter

    none from vector. Insert contains native promoter…

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLN34 - pBait-5GC[1xMS2]-McaS

    Plasmid

    #222403

    Purpose

    arabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

    Depositor

    Katherine Berry

    Article

    Linh D. Nguyen et al Sci Rep 15, 3820 (2025)

    Insert

    McaS (mcaS E. coli)
    

    Use

    Tags

    1xMS2 hairpin, 5GC clamp

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-AAV-syn-FLEX-Volton2-ST-WPRE

    Plasmid

    #172907

    Purpose

    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Has Service

    AAV1

    Insert

    Voltron2-ST
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    hSynapsin1

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid

    #172908

    Purpose

    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Has Service

    AAV1

    Insert

    Ace2N-4AA-mNeon-ST A122D
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    hSynapsin1

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ167

    Plasmid

    #69309

    Purpose

    Gateway entry vector with Cas9p

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    Cas9p (Plant codon-optimized; high GC content at 5 prime region)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBR15.2-2H12.D11opt

    Plasmid

    #221185

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge