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pDGB1_alpha2

Plasmid

#68225

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB1_omega1

Plasmid

#68234

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB1_omega2

Plasmid

#68235

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mYFP

Plasmid

#105144

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mYFP

Tags

81nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMB-AUTr

Plasmid

#61205

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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TAL2369

Plasmid

#36780

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

NCOR1-TALEN_Right (Ncor1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pSB_init-Sb66

Plasmid

#159422

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb66

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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p32m3C Trx-CC

Plasmid

#196475

Purpose

Protein expression of Thiolredoxin-3C cleavage site-CarHCC

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

Trx-CC

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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LI A-B EF1 c2

Plasmid

#54435

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

promoter region of A. thaliana EF1alpha1 /AT1G07940.1

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-mCherry

Plasmid

#135604

Purpose

Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Drp1(A395D)-StrepII

Plasmid

#174430

Purpose

Expresses human Drp1 Isoform 3 with A395D mutation in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

StrepII

Expression

Bacterial

Mutation

A395D

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Drp1-StrepII

Plasmid

#174428

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTT135

Plasmid

#207924

Purpose

Premade UBI10 driven expression of LhG4 (pUBQ10:GR-LhG4:tHSP18.2M) for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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C234-E26: attB2r-Halotag-attB3

Plasmid

#162915

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Halotag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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LI F-G Kan

Plasmid

#104523

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Kanamycin resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-17

Plasmid

#119996

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-ZFP809(1-353)

Plasmid

#67788

Purpose

Mammalian expression of 1-353 amino acids of ZFP809 tagged with Flag

Depositor

Stephen Goff

Article

Wolf et al Nature. 2009 Apr 30;458(7242):1201-4. do

Insert

ZFP809 (Zfp809 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
C453-E14: attB4-EF1p>-attB5

Plasmid

#162975

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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TagBFP2-C1-FKB-PI-PLCN187

Plasmid

#139307

Purpose

N-terminal half of split PI-PLC

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

TagBFP2:FKBP1A(3-108):[GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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iRFP-N1-PI-PLCC100-FRB

Plasmid

#139306

Purpose

C-terminal half of split PI-PLC

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

L. monocytogenes PI-PLC(188-287):IGTAGPRSANS[GA]4: MTOR(2021-2113):iRFP

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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