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pRS314-prp16-101

Plasmid

#89973

Purpose

prp16-101 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

prp16-101 allele (G373S)

Availability

Academic Institutions and Nonprofits only

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pSL3 - Brr2 del113 N1104L

Plasmid

#90253

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 113 amino acids and amino acid N110…

Availability

Academic Institutions and Nonprofits only

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pSL7 - Brr2 del247 N1104L

Plasmid

#90257

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids and N1104L

Availability

Academic Institutions and Nonprofits only
pSL11 - Brr2 del422 N1104L

Plasmid

#90261

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid N110…

Availability

Academic Institutions and Nonprofits only

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pSL12 - Brr2 del422 Q904E

Plasmid

#90262

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid Q904E

Availability

Academic Institutions and Nonprofits only

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pSL13 - Brr2 del422 R1107L

Plasmid

#90263

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid R110…

Availability

Academic Institutions and Nonprofits only

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CaMCLC-His

Plasmid

#111292

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

(S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase

Tags

His tag, GGGSGGGSLEHHHHHH

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA1

Plasmid

#111296

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine MUT

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA3

Plasmid

#111297

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA3 against murine MUT

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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NaVSp1 FL DeltaCTD pIRES

Plasmid

#128077

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel. Residues 1-224.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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NaVAe1 FL DeltaCTD pIRES

Plasmid

#128078

Purpose

Mammalian cell expression vector for NaVAe1 bacterial sodium channel. Residues 1-241.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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NaVAe1 DeltaCC pIRES

Plasmid

#128086

Purpose

Mammalian cell expression vector for NaVAe1 1-264 chimera bacterial sodium channel.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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enz.043-SETD7-Y305F

Plasmid

#154045

Purpose

Expresses SETD7 (Y305F) in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone-lysine N-methyltransferase SETD7 (SETD7 Human)

Tags

His, MBP

Expression

Bacterial

Mutation

changed tyrosine 305 to phenylalanine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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WT Ecm2

Plasmid

#169922

Purpose

Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2

Expression

Bacterial and Yeast

Availability

Academic Institutions and Nonprofits only

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Nuclear PancACe

Plasmid

#215706

Purpose

Expresses acetyl-CoA biosensor in the nucleus

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Mitochondrial PancACe

Plasmid

#215710

Purpose

Expresses acetyl-CoA biosensor in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic cpGFP

Plasmid

#215709

Purpose

Expresses cpGFP in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Mitochondrial cpGFP

Plasmid

#215711

Purpose

Expresses cpGFP in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Nuclear cpGFP

Plasmid

#215707

Purpose

Expresses cpGFP in the nucleus

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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