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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 79,217 results for: rest

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Showing: 6201 - 6220 of 79217 results

  2. pX-evoCas9

    Plasmid
    #107550
    Purpose
    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells
    Depositor
    Anna Cereseto
    Publication
    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    M495V/Y515N/K526E/R661Q
    Promoter
    CBh
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107550-plasmid-map-sequence-id-206015

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  4. PX852

    Plasmid
    #62884
    Purpose
    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62884-plasmid-map-sequence-id-105906

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  6. pcDNA3-K-CAAX

    Plasmid
    #41644
    Purpose
    Expresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box)
    Depositor
    Michael Lin
    Publication
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dronpa145K-CAAX
    Tags
    CAAX box
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    41644-plasmid-map-sequence-id-55896

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  10. pMAH-POLY

    Plasmid
    #64915
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Publication
    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-
    Inserts
    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    Tags
    None
    Expression
    Mammalian
    Promoter
    CMV and U6/H1
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64915-plasmid-map-sequence-id-142448

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  16. eSrtA(2A-9) pET29b

    Plasmid
    #75145
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity
    Depositor
    David Liu
    Publication
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S102C A104H E105D K138P K152I N160K K162H T164N K…
    Promoter
    T7
    Available since
    July 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75145-plasmid-map-sequence-id-466935

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  18. pcDNA3-N-I-N-CAAX

    Plasmid
    #41686
    Purpose
    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation
    Depositor
    Michael Lin
    Publication
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    Tags
    CAAX box
    Expression
    Mammalian
    Mutation
    K145N on Dronpa
    Promoter
    CMV
    Available since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41686-plasmid-map-sequence-id-55893

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Showing: 6201 - 6220 of 79217 results