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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,067 results for: jun

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Showing: 6241 - 6260 of 16067 results

  1. p15a_AcCas12n

    Plasmid
    #203806
    Purpose
    p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.
    Depositor
    Quanjiang Ji
    Publication
    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcCas12n
    Use
    CRISPR
    Promoter
    rpsl
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203806-plasmid-map-sequence-id-405078

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  4. 3322_pETcon_SARS2_Omicron-BA2

    Plasmid
    #184409
    Purpose
    yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD
    Depositor
    Jesse Bloom
    Publication
    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1
    Insert
    SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    Tags
    HA and c-Myc
    Expression
    Yeast
    Mutation
    G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184409-plasmid-map-sequence-id-362502

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  5. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

    Plasmid
    #170198
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)
    Depositor
    Dominic Esposito
    Publication
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    Tags
    His6
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170198-plasmid-map-sequence-id-334260

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  7. R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

    Plasmid
    #170201
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)
    Depositor
    Dominic Esposito
    Publication
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)
    Tags
    His6
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV51
    Available since
    Feb. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    170201-plasmid-map-sequence-id-334264

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  9. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid
    #172828
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172828-plasmid-map-sequence-id-341092

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  10. pX330_VCL sgRNA / hSpCas9

    Plasmid
    #172837
    Purpose
    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172837-plasmid-map-sequence-id-340906

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  11. pαH-S-GSAS-B.1.1.7

    Plasmid
    #171747
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171747-plasmid-map-sequence-id-337765

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  14. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

    Plasmid
    #157986
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Publication
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157986-plasmid-map-sequence-id-332423

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  15. Direct-seq_lentiGuide-Puro-Loop2-8A8G

    Plasmid
    #157983
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Publication
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Loop2-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157983-plasmid-map-sequence-id-332422

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  16. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid
    #129415
    Purpose
    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB18 HDR template (RAB18 Human)
    Use
    CRISPR and TALEN; Endogenous tagging hdr template
    Tags
    sfGFP
    Expression
    Mammalian
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129415-plasmid-map-sequence-id-254821

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  17. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid
    #126425
    Purpose
    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
    Expression
    Mammalian
    Mutation
    alanine-6 of TdT has been changed to a STOP codon
    Promoter
    pCMV
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126425-plasmid-map-sequence-id-252600

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  18. LSB-hsa-miR-29a-3p

    Plasmid
    #103391
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Publication
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-29a-3p target (MIR29A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103391-plasmid-map-sequence-id-195831

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  19. LSB-hsa-miR-221-3p

    Plasmid
    #103365
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-221-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Publication
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-221-3p target (MIR221 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103365-plasmid-map-sequence-id-195779

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  20. LSB-hsa-miR-221-5p

    Plasmid
    #103366
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Publication
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-221-5p target (MIR221 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103366-plasmid-map-sequence-id-195781

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Showing: 6241 - 6260 of 16067 results